Document Detail


Oligosaccharides of arterial basement membrane-like material studied on rabbit aortic myomedial cells in culture.
MedLine Citation:
PMID:  3079579     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oligosaccharides from myomedial basement membrane-like material were characterized in respect of size, attachment to protein and charge heterogeneity after metabolic labelling with [3H] glucosamine. Arterial basement membrane-like material was isolated from cultures of aortic myomedial cells by a sonication-differential centrifugation technique. Glycopeptides were then obtained by proteinase digestion and Sephadex G-50 chromatography. Alkaline borohydride treatment of the glycopeptides followed by Sephadex G-25 chromatography revealed that 10-14% of the oligosaccharides were released by a beta-eliminative reaction. The molecular weight of the alkali labile carbohydrate units was estimated to be approximately 750. Alkali stable oligosaccharides were released from the glycopeptides by hydrazinolysis. The liberated oligosaccharides were retarded by Sephadex G-50 chromatography corresponding to a molecular weight of 2700 using unit B thyroglobulin oligosaccharides as standard. The chromatographic pattern obtained by anion exchange chromatography of the glycopeptides after neuraminidase treatment showed that a major part of the glycopeptides contain one or more sialic acid residues, but also other negatively charged groups--possibly phosphomannosyl residues were present as suggested by [32PO4(3-)] labelling and concanavalin-A-Sepharose chromatography. Concanavalin-A-Sepharose chromatography combined with alpha- mannosidase treatment and gel filtration suggested that a minor part of the glycopeptides were of high-mannose- type.
Authors:
L Heickendorff; T Ledet
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Publication Detail:
Type:  Corrected and Republished Article; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Thrombosis research     Volume:  48     ISSN:  0049-3848     ISO Abbreviation:  Thromb. Res.     Publication Date:  1987 Oct 
Date Detail:
Created Date:  1988-01-27     Completed Date:  1988-01-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0326377     Medline TA:  Thromb Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  269-78     Citation Subset:  IM    
Affiliation:
University Institute of Pathology, Kommunehospitalet, Aarhus, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta / analysis
Basement Membrane / analysis
Cells, Cultured
Chromatography, Gel
Chromatography, Ion Exchange
Glycopeptides / isolation & purification
Hydrolysis
Muscle, Smooth, Vascular / analysis*
Oligosaccharides / isolation & purification*
Rabbits
Chemical
Reg. No./Substance:
0/Glycopeptides; 0/Oligosaccharides
Comments/Corrections
Corrected and Republished From:
Thromb Res 1986 Oct 1;44(1):55-63

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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