Document Detail


Oligosaccharide-assisted direct immunosensing of small molecules.
MedLine Citation:
PMID:  20459059     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
"Sandwich-type" noncompetitive (immunometric) assays allow for high-sensitivity high-throughput macromolecule sensing and determination but cannot be used on small molecules (haptens). Here, we isolated single-chain Fv fragments from a phage-display library, which bound to complexes of particular haptens (vitamin D and A derivatives) with immobilized beta-cyclodextrin or beta-maltosyl residues, and formed ternary complexes. These scFvs enabled novel "semisandwich-type" immunometric assays of haptens with nanomole-range sensitivities.
Authors:
Norihiro Kobayashi; Hiroyuki Oyama; Iwao Suzuki; Yoshinori Kato; Takeo Umemura; Junichi Goto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  82     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-28     Completed Date:  2010-09-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4333-6     Citation Subset:  IM    
Affiliation:
Kobe Pharmaceutical University, 4-19-1, Motoyama-Kitamachi, Higashinada-ku, Kobe 658-8558, Japan. no-kobay@kobepharma-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Biosensing Techniques / methods*
Carbohydrate Conformation
Cattle
Haptens / analysis,  chemistry,  immunology
High-Throughput Screening Assays
Immunoassay / methods*
Models, Molecular
Oligosaccharides / metabolism*
Serum Albumin, Bovine / metabolism
Single-Chain Antibodies / immunology,  metabolism
beta-Cyclodextrins / chemistry,  metabolism
Chemical
Reg. No./Substance:
0/Haptens; 0/Oligosaccharides; 0/Serum Albumin, Bovine; 0/Single-Chain Antibodies; 0/beta-Cyclodextrins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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