| Oligomers are not the limiting factor in the absorption of DL-2-hydroxy-4-(methylthio)butanoic acid in the chicken small intestine. | |
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MedLine Citation:
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PMID: 16493946 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The methionine hydroxy analogue DL-2-hydroxy-4-(methylthio)butanoic acid (HMB) is commonly used as a supplemental source of methionine in commercial animal diets. The HMB free acid is an aqueous solution that contains 88% product in an equilibrium mixture of monomer, dimer, and polymeric compounds. The present study examines whether the presence of these nonmonomeric forms reduces the absorption of the hydroxy analogue in the chicken small intestine. In vivo and in vitro methodologies were used to compare the intestinal absorption of an HMB product containing mainly monomer (HMB-PCM) with commercial HMB. The results from the in vivo perfusion of the jejunum showed no significant differences between the 2 hydroxy analogue sources in monomer absorption from the intestinal lumen, tissue accumulation, or plasma concentration. The results also indicate that the nonmonomeric forms are hydrolyzed during perfusion. Moreover, monomer tissue accumulation in everted sacs showed no significant differences between substrates, either in the presence or in the absence of a H+-gradient; a higher value was observed in the jejunum and ileum in comparison with the duodenum. Similarly, serosal appearance in H+-gradient conditions did not differ significantly between substrates, and it showed the same regional profile as in tissue accumulation. Oligomer hydrolysis was confirmed in vitro without significant differences between segments. In conclusion, the presence of nonmonomeric forms is not a limiting factor in HMB absorption, apparently because of the hydrolytic capacity of intestinal mucosa, as confirmed by experiments in vivo and in vitro. |
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Authors:
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R Martín-Venegas; J F Soriano-García; M P Vinardell; P A Geraert; R Ferrer |
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Publication Detail:
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Type: Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Poultry science Volume: 85 ISSN: 0032-5791 ISO Abbreviation: Poult. Sci. Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2006-02-23 Completed Date: 2006-04-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0401150 Medline TA: Poult Sci Country: United States |
Other Details:
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Languages: eng Pagination: 56-63 Citation Subset: IM |
Affiliation:
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Department de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed* Animals Chickens / metabolism* Diet Hydrogen-Ion Concentration Intestinal Absorption* Intestine, Small / metabolism* Male Methionine / administration & dosage, analogs & derivatives*, chemistry, metabolism |
| Chemical | |
Reg. No./Substance:
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583-91-5/alpha-hydroxy-gamma-methylmercaptobutyric acid; 63-68-3/Methionine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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