Document Detail


Oligomeric cholates: amphiphilic foldamers with nanometer-sized hydrophilic cavities.
MedLine Citation:
PMID:  16351121     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The hydroxyl at the C-3 of cholic acid was converted to an amino group, and the resulting amino-functionalized cholic acid was used as a monomer to prepare amide-linked oligomeric cholates. These cholate oligomers fold into helical structures with nanometer-sized hydrophilic internal cavities in solvent mixtures consisting of mostly nonpolar solvents such as carbon tetrachloride or ethyl acetate/hexane and 2-5% of a polar solvent such as methanol or DMSO. The conformations of the foldamers were studied by UV, fluorescence, fluorescence quenching, and fluorescence resonance energy transfer. The nature of the polar/nonpolar solvents and their miscibility strongly influenced the folding reaction. Folding was cooperative, as evidenced by the sigmoidal curves in solvent denaturation experiments. The folded conformers became more stable with an increase in the chain length. The folding/unfolding equilibrium was highly sensitive toward the amount of polar solvent. One percent variation in the solvent composition could change the folding free energies by 0.5-1.4 kcal/mol.
Authors:
Yan Zhao; Zhenqi Zhong
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  127     ISSN:  0002-7863     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-14     Completed Date:  2006-03-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  17894-901     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Iowa State University, Ames, Iowa 50011-3111, USA. zhaoy@iastate.edu
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MeSH Terms
Descriptor/Qualifier:
Amides / chemistry
Cholates / chemical synthesis,  chemistry*
Fluorescence Resonance Energy Transfer
Molecular Conformation
Nanostructures / chemistry*
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Chemical
Reg. No./Substance:
0/Amides; 0/Cholates

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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