Document Detail


Oligodeoxynucleotide methods for analyzing the circadian clock in the suprachiasmatic nucleus.
MedLine Citation:
PMID:  15817314     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The recent identification of specific genes responsible for the generation of endogenous circadian rhythmicity in the suprachiasmatic nucleus presents a new level of investigation into endogenous rhythmicity and mechanisms of synchronization of this circadian clock with the environmental light?dark cycle. This article describes techniques that employ antisense and decoy oligodeoxynucleotides (ODN) to determine the roles of specific molecular substrates both in endogenous rhythmicity and in regulating the effects of light on the mammalian circadian clock. Application of antisense ODN technology has revealed a role for timeless (Tim) in the core clock mechanism and established that induction of period1 (Per1) is required for light responsiveness. Likewise, a decoy ODN designed to sequester activated CREB protein definitively demonstrated a requirement for CRE-mediated transcription in light signaling. Experiments designed with these molecular tools offer new insights on the interaction of cellular processes and signaling with the molecular clockworks.
Authors:
Shelley A Tischkau; Martha U Gillette
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Methods in enzymology     Volume:  393     ISSN:  1557-7988     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2005  
Date Detail:
Created Date:  2005-04-08     Completed Date:  2008-11-26     Revised Date:  2014-09-20    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  593-610     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Behavior, Animal / physiology
Biological Clocks / genetics,  physiology*,  radiation effects
Cell Cycle Proteins / physiology
Circadian Rhythm / genetics,  physiology*,  radiation effects
Cyclic AMP Response Element-Binding Protein / physiology
Eye Proteins / physiology
Intracellular Signaling Peptides and Proteins / physiology
Mice
Motor Activity
Oligodeoxyribonucleotides, Antisense / metabolism*
Period Circadian Proteins
Photic Stimulation
RNA, Small Interfering / metabolism
Suprachiasmatic Nucleus / physiology*
Grant Support
ID/Acronym/Agency:
HL67007/HL/NHLBI NIH HHS; NS22155/NS/NINDS NIH HHS; NS35859/NS/NINDS NIH HHS; R01 HL067007/HL/NHLBI NIH HHS; R01 NS022155/NS/NINDS NIH HHS; R01 NS035859/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Cyclic AMP Response Element-Binding Protein; 0/Eye Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/Oligodeoxyribonucleotides, Antisense; 0/Per1 protein, mouse; 0/Period Circadian Proteins; 0/RNA, Small Interfering; 0/Timeless protein, mouse

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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