Document Detail

Oligodendrocyte killing by quinolinic acid in vitro.
MedLine Citation:
PMID:  11277985     Owner:  NLM     Status:  MEDLINE    
Quinolinic acid, which is produced by macrophages and microglia, can kill neurons in vivo and in vitro. To test whether quinolinic acid is toxic to oligodendrocytes, glial cells cultured from the brains of 2-day-old rats were incubated with quinolinic acid at concentrations known to kill neurons. The cells were then fixed and immunostained with MAbO4 to mark immature and mature oligodendrocytes and anti-myelin basic protein (MBP) to mark mature oligodendrocytes. The data indicated up to 54% reductions in the numbers of O4-positive cells in cultures after incubation with quinolinic acid. Apoptosis of O4-positive cells began during the first 6 h, and some of the apoptotic cells became fragmented. Further apoptosis, and clumping of dead MBP-positive oligodendrocytes, occurred during longer incubation with quinolinic acid. Thus, quinolinic acid arising from macrophages and microglia during autoimmune disease may take part in a mechanism of oligodendrocyte injury and killing.
W Cammer
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research     Volume:  896     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-30     Completed Date:  2001-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  157-60     Citation Subset:  IM    
Department of Neurology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, F-140, Bronx, NY 10461, USA.
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MeSH Terms
Apoptosis / drug effects*
Cells, Cultured
Oligodendroglia / cytology*,  drug effects*
Quinolinic Acid / pharmacology*
Reg. No./Substance:
89-00-9/Quinolinic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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