Document Detail

Olfactory receptor trafficking involves conserved regulatory steps.
MedLine Citation:
PMID:  11060288     Owner:  NLM     Status:  MEDLINE    
Olfactory receptors are difficult to functionally express in heterologous cells. They are typically retained in the endoplasmic reticulum of cells commonly used for functional expression studies and are only released to the plasma membrane in mature cells of the olfactory receptor neuron lineage. A recently developed olfactory cell line, odora, traffics olfactory receptors to the plasma membrane when differentiated. We found that undifferentiated odora cells do not traffic olfactory receptors to their surface, even though they release the receptors to the Golgi apparatus and endosomes. This behavior differs from other cell lines tested thus far. Differentiated odora cells also properly traffic vomeronasal receptors of the VN1 type, which lack sequence similarity to olfactory receptors. ODR-4, a protein that is necessary for plasma membrane trafficking of a chemosensory receptor in nematodes, facilitates trafficking of rat olfactory receptor U131 in odora and Chinese hamster ovary cells. Olfactory receptor trafficking from the endoplasmic reticulum to the plasma membrane involves at least two steps whose regulation depends on the maturation state of cells in the olfactory receptor neuron lineage. These results also indicate that some components of the regulatory mechanism are conserved.
A A Gimelbrant; S L Haley; T S McClintock
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2000-11-01
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  276     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-05-25     Completed Date:  2001-07-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7285-90     Citation Subset:  IM    
Department of Physiology, University of Kentucky Medical Center, Lexington 40536, USA.
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MeSH Terms
CHO Cells
Caenorhabditis elegans Proteins*
Cell Differentiation
Cell Line
Cell Membrane / metabolism
Endoplasmic Reticulum / metabolism
Endosomes / metabolism
Golgi Apparatus / metabolism
Microscopy, Confocal
Models, Biological
Olfactory Receptor Neurons / metabolism*
Protein Transport
Receptors, Odorant / metabolism*,  physiology
Recombinant Proteins / metabolism
Grant Support
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/ODR-4 protein, C elegans; 0/Receptors, Odorant; 0/Recombinant Proteins

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