Document Detail


Olfactory receptor neuron responses coding for rapid odour sampling.
MedLine Citation:
PMID:  21486768     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vertebrate olfactory receptor neurons (ORNs) are stimulated in a rhythmic manner in vivo, driven by delivery of odorants to the nasal cavity carried by the inhaled air, making olfaction a sense where animals can control the frequency of stimulus delivery. How ORNs encode repeated stimulation at resting, low breathing frequencies and at increased sniffing frequencies is not known, nor is it known if the olfactory transduction cascade is accurate and fast enough to follow high frequency stimulation. We investigated mouse olfactory responses to stimulus frequencies mimicking odorant exposure during low (2Hz) and high (5Hz) frequency sniffing. ORNs reliably follow low frequency stimulations with high fidelity by generating bursts of action potentials at each stimulation at intermediate odorant concentrations, but fail to do so at high odorant concentrations. Higher stimulus frequencies across all odorant concentrations reduced the likelihood of action potential generation, increased the latency of response, and decreased there liability of encoding the onset of stimulation. Thus an increase in stimulus frequency degrades and at high odorant concentrations entirely prevents action potential generation in individual ORNs, causing reduced signalling to the olfactory bulb. These results demonstrate that ORNs do not simply relay timing and concentration of an odorous stimulus, but also process and modulate the stimulus in a frequency-dependent manner which is controlled by the chosen sniffing rate.
Authors:
Ambarish S Ghatpande; Johannes Reisert
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-02-28
Journal Detail:
Title:  The Journal of physiology     Volume:  589     ISSN:  1469-7793     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-02     Completed Date:  2011-08-30     Revised Date:  2012-05-01    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2261-73     Citation Subset:  IM    
Affiliation:
Monell Chemical Senses Center, Philadelphia, PA 19104, USA.
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MeSH Terms
Descriptor/Qualifier:
Action Potentials
Animals
Electric Stimulation
Green Fluorescent Proteins / biosynthesis,  genetics
Inhalation Exposure
Mice
Mice, 129 Strain
Mice, Transgenic
Nasal Mucosa / innervation*
Neural Pathways / physiology
Odors*
Olfactory Nerve / physiology*
Reaction Time
Respiratory Rate*
Sensory Thresholds
Signal Transduction*
Smell*
Time Factors
Grant Support
ID/Acronym/Agency:
DC009613/DC/NIDCD NIH HHS; R01 DC009613-03/DC/NIDCD NIH HHS
Chemical
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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