Document Detail


Olfactory marker protein (OMP) gene deletion causes altered physiological activity of olfactory sensory neurons.
MedLine Citation:
PMID:  8790421     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Olfactory marker protein (OMP) is an abundant, phylogentically conserved, cytoplasmic protein of unknown function expressed almost exclusively in mature olfactory sensory neurons. To address its function, we generated OMP-deficient mice by gene targeting in embryonic stem cells. We report that these OMP-null mice are compromised in their ability to respond to odor stimull, providing insight to OMP function. The maximal electroolfactogram response of the olfactory neuroepithelium to several odorants was 20-40% smaller in the mutants compared with controls. In addition, the onset and recovery kinetics following isoamyl acetate stimulation are prolonged in the null mice. Furthermore, the ability of the mutants to respond to the second odor pulse of a pair is impaired, over a range of concentrations, compared with controls. These results imply that neural activity directed toward the olfactory bulb is also reduced. The bulbar phenotype observed in the OMP-null mouse is consistent with this hypothesis. Bulbar activity of tyrosine hydroxylase, the rate limiting enzyme of catecholamine biosynthesis, and content of the neuropeptide cholecystokinin are reduced by 65% and 50%, respectively. This similarity to postsynaptic changes in gene expression induced by peripheral olfactory deafferentation or naris blockade confirms that functional neural activity is reduced in both the olfactory neuroepithelium and the olfactory nerve projection to the bulb in the OMP-null mouse. These observations provide strong support for the conclusion that OMP is a novel modulatory component of the odor detection/signal transduction cascade.
Authors:
O I Buiakova; H Baker; J W Scott; A Farbman; R Kream; M Grillo; L Franzen; M Richman; L M Davis; S Abbondanzo; C L Stewart; F L Margolis
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  93     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1996-10-24     Completed Date:  1996-10-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  9858-63     Citation Subset:  IM    
Affiliation:
Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cell Line
Electrophysiology
Female
Gene Deletion*
Male
Mice
Molecular Sequence Data
Nerve Tissue Proteins / genetics*,  physiology
Neurons, Afferent / physiology*
Olfactory Bulb / cytology,  physiology*
Olfactory Marker Protein
Restriction Mapping
Chemical
Reg. No./Substance:
0/Nerve Tissue Proteins; 0/Olfactory Marker Protein; 0/Omp protein, mouse
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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