Document Detail


Olfactory ensheathing cells promote neurite extension from embryonic olfactory receptor cells in vitro.
MedLine Citation:
PMID:  9890625     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of ensheathing cells, a macroglial cell type with a unique presence in the olfactory system, in the outgrowth of olfactory receptor cell neurites was explored in vitro. Glial cell cultures harvested from both the olfactory bulb nerve layer and the hippocampus were established and immunocytochemically characterized. The expression of the p75 low-affinity nerve growth factor receptor by ensheathing cells was used to distinguish them from other macroglial subpopulations. Results indicated that ensheathing cell cultures were approximately 80% pure. Olfactory receptor cells were cocultured with ensheathing or hippocampal glial cells or were seeded on laminin or poly-L-lysine as controls. Olfactory receptor cells extended the longest primary neurites when cocultured with ensheathing cells. Neurite extension on hippocampal glia and laminin was less extensive than that observed on ensheathing cells but higher than that on poly-L-lysine. The neurite outgrowth-promoting effect of ensheathing cells was, at least in part, mediated by diffusible factors, because olfactory receptor cell neurite extension could also be facilitated when receptor cells were cultured in ensheathing cell-conditioned media. In contrast, cortical neurons extended neurites of equivalent lengths on ensheathing and hippocampal glia. The results suggest that ensheathing cells may release factors that support the continuous outgrowth of olfactory receptor cell axons and, therefore, the capacity of this pathway to recover from injury.
Authors:
K W Kafitz; C A Greer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Glia     Volume:  25     ISSN:  0894-1491     ISO Abbreviation:  Glia     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-03-29     Completed Date:  1999-03-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8806785     Medline TA:  Glia     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  99-110     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery and Section of Neurobiology, Yale University School of Medicine, New Haven, Connecticut 06520-8039, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Count
Cells, Cultured
Hippocampus / cytology,  embryology
Immunohistochemistry
Nerve Growth Factors / physiology
Neurites / physiology*,  ultrastructure
Olfactory Bulb / cytology*,  embryology,  ultrastructure
Olfactory Pathways / cytology
Olfactory Receptor Neurons / physiology*,  ultrastructure
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
DC00210/DC/NIDCD NIH HHS; NS10174/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Nerve Growth Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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