Document Detail


Oleuropein, an antioxidant polyphenol from olive oil, is poorly absorbed from isolated perfused rat intestine.
MedLine Citation:
PMID:  11110859     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Epidemiological studies have shown that the incidence of heart disease and certain cancers is lower in the Mediterranean region. This has been attributed to the high consumption of olive oil in the Mediterranean diet, which contains polyphenolic compounds with antioxidant activity. Although many in vitro studies have been performed to elucidate mechanisms by which these compounds may act, there are virtually no data relating to their fate after ingestion. Therefore, we decided to investigate the intestinal absorption of one of the major olive oil polyphenolics, oleuropein. To do this, a novel in situ intestinal perfusion technique was developed, and the absorption of oleuropein was studied under both iso-osmotic and hypotonic luminal conditions. Oleuropein was absorbed, with an apparent permeability coefficient (P:(app)) of 1.47 +/- 0.13 x 10(-6) cm/s (+/-SE) observed under iso-osmotic conditions. The mechanism of absorption is unclear but may involve transcellular transport (SGLT1) or paracellular movement. Under hypotonic conditions, the permeability of oleuropein was significantly greater (5.92 +/- 0.49 x 10(-6) cm/s, P: < 0.001). This increase is thought to be due to an increase in paracellular movement facilitated by the opening of paracellular junctions in response to hypotonicity. Overall, we determined that the olive oil polyphenolic oleuropein can be absorbed, albeit poorly, from isolated perfused rat intestine. Therefore, it is possible that it or its metabolites may confer a positive health benefit after the consumption of olive oil, most likely via an antioxidant mechanism.
Authors:
S C Edgecombe; G L Stretch; P J Hayball
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of nutrition     Volume:  130     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-01-10     Completed Date:  2001-02-01     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2996-3002     Citation Subset:  IM    
Affiliation:
Centre for Pharmaceutical Research, University of South Australia, North Terrace, Adelaide, South Australia, 5000, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / therapeutic use*
Biological Availability
Flavonoids*
Hypotonic Solutions
Intestinal Absorption*
Membrane Glycoproteins / physiology
Models, Animal
Monosaccharide Transport Proteins / physiology
Permeability
Phenols / chemistry,  pharmacokinetics*
Plant Oils / analysis,  metabolism,  pharmacokinetics*
Polymers / chemistry,  pharmacokinetics*
Pyrans / metabolism,  pharmacokinetics*
Rats
Sodium-Glucose Transporter 1
Time Factors
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Flavonoids; 0/Hypotonic Solutions; 0/Membrane Glycoproteins; 0/Monosaccharide Transport Proteins; 0/Phenols; 0/Plant Oils; 0/Polymers; 0/Pyrans; 0/Slc5a1 protein, rat; 0/Sodium-Glucose Transporter 1; 0/polyphenols; 32619-42-4/oleuropein; 8001-25-0/olive oil

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