Document Detail


Oleic acid uptake by in vitro English sparrow skeletal muscle.
MedLine Citation:
PMID:  16432889     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Studies of prolonged avian flight have shown it to require large amounts of energy supplied mainly by free fatty acids (FFA). In the present study, the high levels of plasma ketone bodies found in sparrows (2.58 mmol l(-1)) are supportive of the use of fatty acids for flight. To determine the nature of fatty acid (oleic acid, OA) uptake, various pharmacological agents were used. The uptake of OA was examined using the extensor digitorum communis (EDC) muscle of English sparrows incubated in vitro. Initial studies demonstrated that radiolabeled OA uptake decreased in the presence of increasing unlabeled OA, suggesting that uptake occurred by a facilitative transport process. To further characterize OA uptake, EDC muscles were incubated with either: insulin (2 ng ml(-1)), insulin-like growth factor isoform-1 (IGF-I; 48 ng ml(-1)), 5'-Aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR; 2 mmol) or caffeine (5 mmol). Insulin, but not IGF-I, significantly increased OA uptake by avian EDC (P < 0.01). Caffeine and AICAR were ineffective at increasing OA uptake. A specific inhibitor of FFA transport by fatty acid transporters (FAT/CD36), sulfo-N-succinimidyl oleate (SSO; 500 micromoles), significantly decreased OA uptake at 2.5 min. The effectiveness of SSO suggests that a FAT/CD36-like protein is expressed in avian tissues. As uptake of OA was not completely blocked by SSO, it is likely that other mechanisms for FFA movement across membranes, such as diffusion, may be present.
Authors:
Karen L Sweazea; Eldon J Braun
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of experimental zoology. Part A, Comparative experimental biology     Volume:  305     ISSN:  1548-8969     ISO Abbreviation:  J. Exp. Zoolog. Part A Comp. Exp. Biol.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-01-30     Completed Date:  2006-04-26     Revised Date:  2009-10-05    
Medline Journal Info:
Nlm Unique ID:  101168223     Medline TA:  J Exp Zool A Comp Exp Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  268-76     Citation Subset:  IM    
Copyright Information:
Copyright 2006 Wiley-Liss, Inc.
Affiliation:
Department of Physiology, Arizona Health Sciences Center, University of Arizona, Tucson, Arizona 85724-5051, USA.
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MeSH Terms
Descriptor/Qualifier:
Aminoimidazole Carboxamide / analogs & derivatives,  pharmacology
Animals
Biological Transport / drug effects
Caffeine / pharmacology
Fatty Acid Transport Proteins / antagonists & inhibitors,  metabolism*
Flight, Animal / physiology*
Insulin / pharmacology
Insulin-Like Growth Factor I / pharmacology
Ketone Bodies / blood
Muscle, Skeletal / metabolism*
Oleic Acid / metabolism*
Oleic Acids / pharmacology
Ribonucleotides / pharmacology
Sparrows / blood,  metabolism*
Succinimides / pharmacology
Chemical
Reg. No./Substance:
0/Fatty Acid Transport Proteins; 0/Ketone Bodies; 0/Oleic Acids; 0/Ribonucleotides; 0/Succinimides; 0/sulfo-N-succinimidyl oleate; 11061-68-0/Insulin; 112-80-1/Oleic Acid; 3031-94-5/AICA ribonucleotide; 360-97-4/Aminoimidazole Carboxamide; 58-08-2/Caffeine; 67763-96-6/Insulin-Like Growth Factor I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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