Document Detail

Oleic acid synthesized in the periventricular zone promotes axonogenesis in the striatum during brain development.
MedLine Citation:
PMID:  20626566     Owner:  NLM     Status:  MEDLINE    
Our previous works showed that oleic acid synthesized in vitro by astrocytes in response to albumin behaves as a neurotrophic factor in neurons, up-regulating several proteins, such as the axonal growth marker growth-associated protein 43(GAP-43). Although the molecular mechanism of this process is fairly known, there is no evidence pinpointing the region/s in which oleic acid is synthesized. In this study, we show that the rate-limiting enzyme in oleic acid synthesis, stearoyl-CoA desaturase (SCD-1), is located in the periventricular zone of the brain of newborn rats, simultaneously to an increase in the amount of free oleic acid in the forebrain. In addition, the spatio-temporal presence of albumin - the signal that promotes oleic acid synthesis - and that of GAP-43 are correlated with that of SCD-1. Using organotypic slice cultures, we found that albumin up-regulates SCD-1 and stimulates the growth of GAP-43-positive axons in the striatum. The effect of albumin on GAP-43 was reduced when SCD-1 was silenced by siRNA. In conclusion, our results suggest that albumin up-regulates axonogenesis in the striatum by increasing the amount of the neurotrophic factor oleic acid synthesized by SCD-1 in the periventricular zone of the newborn brain.
Erica Polo-Hernández; Fernando De Castro; Alejandro G García-García; Arantxa Tabernero; José M Medina
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-03
Journal Detail:
Title:  Journal of neurochemistry     Volume:  114     ISSN:  1471-4159     ISO Abbreviation:  J. Neurochem.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-24     Completed Date:  2010-10-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  1756-66     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors. Journal Compilation © 2010 International Society for Neurochemistry.
Departamento de Bioquímica y Biología Molecular, Instituto de Neurociencias de Castilla y León, INCYL, Universidad de Salamanca, Salamanca, Spain.
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MeSH Terms
Albumins / metabolism
Animals, Newborn
Axons / physiology*
Cerebral Ventricles / growth & development,  metabolism*,  ultrastructure
Corpus Striatum / growth & development,  metabolism*,  ultrastructure
GAP-43 Protein / biosynthesis
Gene Expression Regulation, Developmental
Oleic Acid / biosynthesis*
RNA, Small Interfering / genetics
Rats, Wistar
Stearoyl-CoA Desaturase / genetics,  metabolism
Tissue Culture Techniques
Reg. No./Substance:
0/Albumins; 0/GAP-43 Protein; 0/RNA, Small Interfering; 112-80-1/Oleic Acid; EC Desaturase

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