Document Detail


Oleic acid is a key cytotoxic component of HAMLET-like complexes.
MedLine Citation:
PMID:  22628302     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Abstract HAMLET is a complex of α-lactalbumin (α-LA) with oleic acid (OA) that selectively kills tumor cells and Streptococcus pneumoniae. To assess the contribution of the proteinaceous component to cytotoxicity of HAMLET, OA complexes with proteins structurally and functionally distinct from α-LA were prepared. Similar to HAMLET, the OA complexes with bovine β-lactoglobulin (bLG) and pike parvalbumin (pPA) (bLG-OA-45 and pPA-OA-45, respectively) induced S. pneumoniae D39 cell death. The activation mechanisms of S. pneumoniae death for these complexes were analogous to those for HAMLET, and the cytotoxicity of the complexes increased with OA content in the preparations. The half-maximal inhibitory concentration for HEp-2 cells linearly decreased with rise in OA content in the preparations, and OA concentration in the preparations causing HEp-2 cell death was close to the cytotoxicity of OA alone. Hence, the cytotoxic action of these complexes against HEp-2 cells is induced mostly by OA. Thermal stabilization of bLG upon association with OA implies that cytotoxicity of bLG-OA-45 complex cannot be ascribed to molten globule-like conformation of the protein component. Overall, the proteinaceous component of HAMLET-like complexes studied is not a prerequisite for their activity; the cytotoxicity of these complexes is mostly due to the action of OA.
Authors:
Sergei E Permyakov; Ekaterina L Knyazeva; Leysan M Khasanova; Roman S Fadeev; Andrei P Zhadan; Hazeline Roche-Hakansson; Anders P Håkansson; Vladimir S Akatov; Eugene A Permyakov
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological chemistry     Volume:  393     ISSN:  1437-4315     ISO Abbreviation:  Biol. Chem.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-05-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9700112     Medline TA:  Biol Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  85-92     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Protection from Clostridium difficile toxin B-catalysed Rac1/Cdc42 glucosylation by tauroursodeoxych...
Next Document:  Identification of ATF2 as a transcriptional regulator of renin gene.