| Oleic acid inhibits stearic acid-induced inhibition of cell growth and pro-inflammatory responses in human aortic endothelial cells. | |
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MedLine Citation:
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PMID: 20852092 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Saturated fatty acids (SFAs), significant components of both enteral/parenteral nutritional formulations (including diet), are linked to cardiovascular disease complications, such as atherosclerosis. We investigated whether oleic acid (C18:1n-9) reduces the growth inhibitory and pro-inflammatory effects of the stearic acid (C18:0) in human aortic endothelial cells (HAEC). Stearic acid induced growth inhibition at concentrations less than 50 μM, whereas higher concentrations invoked cytotoxicity. Stearic acid-induced growth inhibition and cytotoxic effects were eradicated upon cosupplementation with oleic acid (25 μM). Oleic acid (as low as 5 μM) also inhibited the stearic acid-induced increase in intercellular adhesion molecule-1 (ICAM-1) expression. Stearic acid-induced phosphorylation of nuclear factor-kappa B (NF-κB), a transcriptional regulator of ICAM-1, was also reduced by oleic acid. HAECs supplemented with either stearic or oleic acid resulted in cellular incorporation of C18:0 and C18:1n-9, respectively. Stearic acid primarily incorporated into phospholipids without increasing the total fatty acid content in HAECs. In contrast, oleic acid, with or without stearic acid, incorporated into both phospholipids and triglycerides, with a significant increase in total fatty acid amounts in triglycerides. Our data suggest that oleic acid has the ability to reduce the inflammatory effects of long-chain SFAs in HAECs through reducing cellular stearic acid incorporation and NF-κB activation. |
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Authors:
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Kevin A Harvey; Candace L Walker; Zhidong Xu; Phillip Whitley; Thomas M Pavlina; Mary Hise; Gary P Zaloga; Rafat A Siddiqui |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-17 |
Journal Detail:
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Title: Journal of lipid research Volume: 51 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-10 Completed Date: 2011-02-22 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 3470-80 Citation Subset: IM |
Affiliation:
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Cellular Biochemistry Laboratory, Methodist Research Institute, Indianapolis, IN, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aorta
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cytology,
drug effects*,
metabolism Apoptosis / drug effects Cell Proliferation / drug effects* Cells, Cultured Dietary Fats, Unsaturated / pharmacology, therapeutic use* Dose-Response Relationship, Drug Endothelial Cells / drug effects*, metabolism Humans Inflammation / chemically induced, drug therapy*, metabolism Intercellular Adhesion Molecule-1 / metabolism Lipid Metabolism / drug effects NF-kappa B / metabolism Oleic Acid / pharmacology, therapeutic use* Stearic Acids / administration & dosage, toxicity* Triglycerides / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Dietary Fats, Unsaturated; 0/NF-kappa B; 0/Stearic Acids; 0/Triglycerides; 112-80-1/Oleic Acid; 126547-89-5/Intercellular Adhesion Molecule-1; 57-11-4/stearic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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