Document Detail

Oleic acid inhibits endothelial nitric oxide synthase by a protein kinase C-independent mechanism.
MedLine Citation:
PMID:  7591016     Owner:  NLM     Status:  MEDLINE    
Many obese hypertensive individuals have a cluster of cardiovascular risk factors. This cluster includes plasma nonesterified fatty acid concentrations and turnover rates that are higher and more resistant to suppression by insulin than in lean and obese normotensive individuals. The higher fatty acids may contribute to cardiovascular risk in these patients by inhibiting endothelial cell nitric oxide synthase activity. To test this hypothesis, we quantified the effects of oleic (18:1[cis]) and other 18-carbon fatty acids on nitric oxide synthase activity in cultured bovine pulmonary artery endothelial cells by measuring the conversion of [3H]L-arginine to [3H]L-citrulline. Oleic acid (from 10 to 100 mumol/L) caused a concentration-dependent decrease in nitric oxide synthase activity at baseline and during ATP and ionomycin (Ca2+ ionophore) stimulation. At 100 mumol/L, linoleic (18:2[cis]) and oleic acids caused similar reductions of nitric oxide synthase activity, whereas elaidic (18:1[trans]) and stearic (18:0) acids had no effect. Oleic acid also inhibited the endothelium-dependent vasodilator response to acetylcholine in rabbit femoral artery rings preconstricted with phenylephrine (P < .05) but had no effect on the response to nitroprusside. The pattern of 18-carbon fatty acid effects on nitric oxide synthase activity in endothelial cells is consistent with activation of protein kinase C. Although oleic acid increased protein kinase C activity in endothelial cells, neither depletion of protein kinase C by 24-hour pretreatment with phorbol 12-myristate 13-acetate nor its inhibition with staurosporine eliminated the inhibitory effect of oleic acid on nitric oxide synthase.(ABSTRACT TRUNCATED AT 250 WORDS)
R K Davda; K T Stepniakowski; G Lu; M E Ullian; T L Goodfriend; B M Egan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Hypertension     Volume:  26     ISSN:  0194-911X     ISO Abbreviation:  Hypertension     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1995-12-07     Completed Date:  1995-12-07     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  764-70     Citation Subset:  IM    
Division of Nephrology, Medical University of South Carolina, Charleston 29425-2251, USA.
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MeSH Terms
Cells, Cultured
Endothelium, Vascular / metabolism*
Enzyme Activation / drug effects
Femoral Artery / drug effects,  physiology
Hypertension / metabolism
Nitric Oxide Synthase / antagonists & inhibitors*
Oleic Acid
Oleic Acids / blood,  pharmacology*
Protein Kinase C / metabolism*
Vasodilation / drug effects
Grant Support
R01-43164//PHS HHS
Reg. No./Substance:
0/Oleic Acids; 112-80-1/Oleic Acid; EC Oxide Synthase; EC Kinase C

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