| Oleic Acid Stimulates System A Amino Acid Transport in Primary Human Trophoblast Cells Mediated by Toll-Like Receptor 4. | |
| | |
MedLine Citation:
|
PMID: 23275648 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Obese women have an increased risk to deliver large babies. However, the mechanisms underlying fetal overgrowth in these pregnancies are not well understood. Obese pregnant women typically have elevated circulating lipid levels. We tested the hypothesis that fatty acids stimulate placental amino acid transport, mediated via toll-like receptor 4 (TLR4) and mammalian target of rapamycin (mTOR) signaling pathways. Circulating non-esterified fatty acid (NEFA) levels and placental TLR4 expression were assessed in women with varying pre-pregnancy body mass index (BMI). The effects of oleic acid on system A and system L amino acid transport, and on the activation of the mTOR (4EBP1, S6K1, rpS6), TLR4 (IĸB, JNK, p38 MAPK), and STAT3 signaling pathways were determined in cultured primary human trophoblast cells. Maternal circulating NEFAs (n=33), but not placental TLR4 mRNA expression (n=16), correlated positively with BMI (P<0.05). Oleic acid increased trophoblast JNK and STAT3 phosphorylation (P<0.05), whereas mTOR activity was unaffected. Furthermore, oleic acid doubled trophoblast system A activity (P<0.05), without affecting system L activity. siRNA mediated silencing of TLR4 expression prevented the stimulatory effect of oleic acid on system A activity. Our data suggests that maternal fatty acids can increase placental nutrient transport via TLR4, thereby potentially affecting fetal growth. |
| | |
Authors:
|
Susanne Lager; Francesca Gaccioli; Vanessa I Ramirez; Helen N Jones; Thomas Jansson; Theresa L Powell |
Related Documents
:
|
22986818 - D-amino acid-induced expression of d-amino acid oxidase in the yeast schizosaccharomyce... 23006568 - Biosynthesis of silver nanoparticles using alternanthera sessilis (linn.) extract and t... 23230388 - Electron detachment dissociation of synthetic heparan sulfate glycosaminoglycan tetrasa... 1668558 - Nmr studies of melanins: characterization of a soluble melanin free acid from sepia ink. 16132128 - An infrared spectroscopic method for quantitative analysis of fatty alcohols and fatty ... 10457478 - Determination of trace anions in concentrated weak acids by ion chromatography. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-12-30 |
Journal Detail:
|
Title: Journal of lipid research Volume: - ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2012 Dec |
Date Detail:
|
Created Date: 2012-12-31 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
University of Texas Health Science Center, San Antonio, United States; |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Prospective Feasibility Study to Evaluate Neoadjuvant-synchronous S-1 + RT for Locally Advanced Rect...
Next Document: What Do You Think Would Make You Happier? What Do You Think You Would Choose?().