| Oil biosynthesis in microsomal membrane preparations from Mortierella alpina. | |
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MedLine Citation:
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PMID: 11171179 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Microsomal membrane preparations from Mortierella alpina catalysed the conversion of sn-glycerol 3-phosphate and [(14)C]oleoyl-CoA to radioactive phosphatidic acid, diacylglycerol and triacylglycerol. Experiments with lysophosphatidic acid and [(14)C]oleoyl-CoA gave a similar pattern of radioactivity in the complex lipids. The specific activity of lysophosphatidate acyltransferase was almost eight times greater than sn-glycerol-3-phosphate acyltransferase, indicating that the first acylation step was limiting in oil assembly in the microsomal membranes. Little conversion of radioactive oleate into phosphatidylcholine occurred, suggesting that triacylglycerol assembly and its relationship to phosphatidylcholine metabolism differed to that found in oilseeds. |
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Authors:
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S Chatrattanakunchai; T Fraser; K Stobart |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical Society transactions Volume: 28 ISSN: 0300-5127 ISO Abbreviation: Biochem. Soc. Trans. Publication Date: 2000 Dec |
Date Detail:
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Created Date: 2001-02-26 Completed Date: 2001-05-31 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7506897 Medline TA: Biochem Soc Trans Country: England |
Other Details:
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Languages: eng Pagination: 707-9 Citation Subset: IM |
Affiliation:
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School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acyl Coenzyme A
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metabolism Acyltransferases / metabolism Carbon Radioisotopes Glycerol-3-Phosphate O-Acyltransferase / metabolism Glycerophosphates / metabolism Intracellular Membranes / metabolism Kinetics Lysophospholipids / metabolism Microsomes / metabolism* Mucorales / metabolism* Oils / metabolism* Phosphatidic Acids / metabolism Phosphatidylcholines / metabolism Radioisotope Dilution Technique |
| Chemical | |
Reg. No./Substance:
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0/Acyl Coenzyme A; 0/Carbon Radioisotopes; 0/Glycerophosphates; 0/Lysophospholipids; 0/Oils; 0/Phosphatidic Acids; 0/Phosphatidylcholines; 1716-06-9/oleoyl-coenzyme A; EC 2.3.-/Acyltransferases; EC 2.3.1.15/Glycerol-3-Phosphate O-Acyltransferase; EC 2.3.1.52/2-acylglycerophosphate acyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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