Document Detail

Oil biosynthesis in microsomal membrane preparations from Mortierella alpina.
MedLine Citation:
PMID:  11171179     Owner:  NLM     Status:  MEDLINE    
Microsomal membrane preparations from Mortierella alpina catalysed the conversion of sn-glycerol 3-phosphate and [(14)C]oleoyl-CoA to radioactive phosphatidic acid, diacylglycerol and triacylglycerol. Experiments with lysophosphatidic acid and [(14)C]oleoyl-CoA gave a similar pattern of radioactivity in the complex lipids. The specific activity of lysophosphatidate acyltransferase was almost eight times greater than sn-glycerol-3-phosphate acyltransferase, indicating that the first acylation step was limiting in oil assembly in the microsomal membranes. Little conversion of radioactive oleate into phosphatidylcholine occurred, suggesting that triacylglycerol assembly and its relationship to phosphatidylcholine metabolism differed to that found in oilseeds.
S Chatrattanakunchai; T Fraser; K Stobart
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical Society transactions     Volume:  28     ISSN:  0300-5127     ISO Abbreviation:  Biochem. Soc. Trans.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-02-26     Completed Date:  2001-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506897     Medline TA:  Biochem Soc Trans     Country:  England    
Other Details:
Languages:  eng     Pagination:  707-9     Citation Subset:  IM    
School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK.
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MeSH Terms
Acyl Coenzyme A / metabolism
Acyltransferases / metabolism
Carbon Radioisotopes
Glycerol-3-Phosphate O-Acyltransferase / metabolism
Glycerophosphates / metabolism
Intracellular Membranes / metabolism
Lysophospholipids / metabolism
Microsomes / metabolism*
Mucorales / metabolism*
Oils / metabolism*
Phosphatidic Acids / metabolism
Phosphatidylcholines / metabolism
Radioisotope Dilution Technique
Reg. No./Substance:
0/Acyl Coenzyme A; 0/Carbon Radioisotopes; 0/Glycerophosphates; 0/Lysophospholipids; 0/Oils; 0/Phosphatidic Acids; 0/Phosphatidylcholines; 1716-06-9/oleoyl-coenzyme A; EC 2.3.-/Acyltransferases; EC O-Acyltransferase; EC acyltransferase

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