Document Detail


Oil biosynthesis in microsomal membrane preparations from Mortierella alpina.
MedLine Citation:
PMID:  11171179     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microsomal membrane preparations from Mortierella alpina catalysed the conversion of sn-glycerol 3-phosphate and [(14)C]oleoyl-CoA to radioactive phosphatidic acid, diacylglycerol and triacylglycerol. Experiments with lysophosphatidic acid and [(14)C]oleoyl-CoA gave a similar pattern of radioactivity in the complex lipids. The specific activity of lysophosphatidate acyltransferase was almost eight times greater than sn-glycerol-3-phosphate acyltransferase, indicating that the first acylation step was limiting in oil assembly in the microsomal membranes. Little conversion of radioactive oleate into phosphatidylcholine occurred, suggesting that triacylglycerol assembly and its relationship to phosphatidylcholine metabolism differed to that found in oilseeds.
Authors:
S Chatrattanakunchai; T Fraser; K Stobart
Related Documents :
6660509 - An affinity ligand for phospholipase a2 purification.
1122309 - The biosynthesis and turnover of different molecular species of rat testicular choline ...
8452869 - Phosphatidic acid stimulates the rolipram-sensitive cyclic nucleotide phosphodiesterase...
10192509 - Antibodies against lysophosphatidylcholine and oxidized ldl in patients with sle.
16563639 - Neuroprotection of rat hippocampal slices exposed to oxygen-glucose deprivation by enri...
3535899 - Glucose-induced phospholipid hydrolysis in isolated pancreatic islets: quantitative eff...
11856549 - Characterization of rat liver bile acid acyl glucuronosyltransferase.
6660509 - An affinity ligand for phospholipase a2 purification.
19202309 - The volatile constituents of the flower, leaf, and stem of verbascum wiedemannianum gro...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical Society transactions     Volume:  28     ISSN:  0300-5127     ISO Abbreviation:  Biochem. Soc. Trans.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-02-26     Completed Date:  2001-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506897     Medline TA:  Biochem Soc Trans     Country:  England    
Other Details:
Languages:  eng     Pagination:  707-9     Citation Subset:  IM    
Affiliation:
School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acyl Coenzyme A / metabolism
Acyltransferases / metabolism
Carbon Radioisotopes
Glycerol-3-Phosphate O-Acyltransferase / metabolism
Glycerophosphates / metabolism
Intracellular Membranes / metabolism
Kinetics
Lysophospholipids / metabolism
Microsomes / metabolism*
Mucorales / metabolism*
Oils / metabolism*
Phosphatidic Acids / metabolism
Phosphatidylcholines / metabolism
Radioisotope Dilution Technique
Chemical
Reg. No./Substance:
0/Acyl Coenzyme A; 0/Carbon Radioisotopes; 0/Glycerophosphates; 0/Lysophospholipids; 0/Oils; 0/Phosphatidic Acids; 0/Phosphatidylcholines; 1716-06-9/oleoyl-coenzyme A; EC 2.3.-/Acyltransferases; EC 2.3.1.15/Glycerol-3-Phosphate O-Acyltransferase; EC 2.3.1.52/2-acylglycerophosphate acyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Accumulation of storage products in oat during kernel development.
Next Document:  New insights into the mechanisms of lipid-body biogenesis in plants and other organisms.