Document Detail


Oil, Fatty Acid, Flavonoid, and Resveratrol Content Variability and FAD2A Functional SNP Genotypes in the U.S. Peanut Mini-Core Collection.
MedLine Citation:
PMID:  23379758     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Peanut seeds contain high amounts of oil and protein as well as some useful bioactive phytochemicals which can contribute to human health. The U.S. peanut mini-core collection is an important genetic resource for improving seed quality and developing new cultivars. Variability of seed chemical composition within the mini-core was evaluated from freshly harvested seeds for two years. Oil, fatty acid composition, and flavonoid/ resveratrol content were quantified by NMR, GC, and HPLC, respectively. Significant variability was detected in seed chemical composition among accessions and botanical varieties. Accessions were further genotyped with a functional SNP marker from the FAD2A gene using real-time PCR and classified into three genotypes with significantly different O/L ratios: wild type (G/G with a low O/L ratio <1.7), heterozygote (G/A with O/L ratio >1.4 but <1.7), and mutant (A/A with a high O/L ratio >1.7). The results from real-time PCR genotyping and GC fatty acid analysis were consistent. Accessions with high amounts of oil, quercetin, high seed weight, and O/L ratio were identified. The results from this study may be useful not only for peanut breeders, food processors, and product consumers to select suitable accessions or cultivars, but also for curators to potentially expand the mini-core collection.
Authors:
Ming Li Wang; Charles Y Chen; Brandon Tonnis; Noelle A Barkley; David L Pinnow; Roy N Pittman; Jerry Davis; C Corley Holbrook; H Thomas Stalker; Gary A Pederson
Related Documents :
8543558 - Stimulation of phagocytosis and phagosome-lysosome fusion by glycosphingolipids from sp...
6270208 - A new species of lysosome in rabbit polymorphonuclear leukocytes.
8838158 - Effects of methylphenidate (ritalin) on mammalian myocardial ultrastructure.
24292338 - 2-(1h-pyrazol-1-yl)acetic acids as chemoattractant receptor-homologous molecule express...
17468208 - Nad-dependent isocitrate dehydrogenase mutants of arabidopsis suggest the enzyme is not...
11101328 - Acetic acid production from lactose by an anaerobic thermophilic coculture immobilized ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-4
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Self-reported depressive symptoms in women hospitalized for acute coronary syndrome.
Next Document:  Can early second-look tympanoplasty reduce the rate of conversion to modified radical mastoidectomy?