Document Detail


Offspring after Embryo-preserving Biopsy of the Embryoblast with Standard ICSI Equipment in Mouse Blastocysts.
MedLine Citation:
PMID:  22021687     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
MATERIALS AND METHODS: A total of 40 blastocysts from a black mouse strain were biopsied. The mouse blastocysts were fixed with a holding pipette. The zona pellucida and trophectoderm layer were penetrated with an injection pipette, and cells from the inner cell mass (ICM) were aspirated. The pipette was removed and the ICM cells were transferred into a medium.
RESULTS: The blastocysts collapsed after pipette removal and were allowed to regenerate for 6 h. Twenty-four blastocysts recovered, expanded and were implanted into four white surrogate mothers. One surrogate mother gave birth to two black pups.
CONCLUSION: This experiment demonstrates that nondestructive blastocyst biopsy from the ICM is possible in mice.
Authors:
Ralf Dittrich; Laura Lotz; Wolfgang Würfel; Dieter Engelkamp; Martina Döhler; Theodoros Maltaris; Inge Hoffmann; Andreas Mueller; Matthias W Beckmann; Patricia G Oppelt
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  In vivo (Athens, Greece)     Volume:  25     ISSN:  1791-7549     ISO Abbreviation:  In Vivo     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-10-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806809     Medline TA:  In Vivo     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  935-9     Citation Subset:  IM    
Affiliation:
D.Sc., Department of Obstetrics and Gynecology, Erlangen University Hospital, University of Erlangen-Nuremberg, Universitaetsstrasse 21-23, D-91054 Erlangen, Germany. ralf.dittrich@uk-erlangen.de.
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