Document Detail

Oestrogen and progesterone receptor binding capacity and oestrogen receptor alpha expression (ERalpha mRNA) along the cervix of cycling ewes.
MedLine Citation:
PMID:  18402754     Owner:  NLM     Status:  MEDLINE    
The aim of the present work was to study the oestrogen receptor (ER) and progesterone receptor (PR) binding capacity and the oestrogen receptor alpha (ERalpha) mRNA concentration in cranial and caudal cervix during the ovine oestrous cycle. Cervical samples of synchronised Corriedale ewes were obtained on Day 1 (n=7), 6 (n=6) or 13 (n=7) after oestrus detection (Day 0). The ER and PR binding capacity by ligand-binding assay and the ERalpha mRNA concentration by solution hybridisation in both cranial and caudal zones of the cervix were determined. The ER and PR binding capacity were higher (P<0.005) on Day 1 than on Days 6 and 13 in both cranial and caudal zones. The ERalpha mRNA concentrations were higher (P<0.0001) on Day 1 than on Days 6 and 13 only in the caudal zone. The PR binding capacity and ERalpha mRNA concentration were higher (P<0.005) in the caudal than in the cranial zone on Day 1. The ER and PR expression in the ovine cervix varied during the oestrous cycle in agreement with the known upregulation exerted by oestrogen and downregulation exerted by progesterone. Differences in ER and PR expression along the longitudinal axis of the ovine cervix were found, reflecting histological and functional differences between the cranial and caudal zones.
M Rodríguez-Piñón; C Tasende; P Puime; E G Garófalo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction, fertility, and development     Volume:  20     ISSN:  1031-3613     ISO Abbreviation:  Reprod. Fertil. Dev.     Publication Date:  2008  
Date Detail:
Created Date:  2008-04-11     Completed Date:  2008-06-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8907465     Medline TA:  Reprod Fertil Dev     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  350-6     Citation Subset:  IM    
Biochemistry, Department of Molecular and Cellular Biology, Faculty of Veterinary, Montevideo, Uruguay.
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MeSH Terms
Cervix Uteri / metabolism*
Estrogen Receptor alpha / biosynthesis
Estrous Cycle / physiology*
Gene Expression
RNA, Messenger / biosynthesis
Receptors, Estrogen / metabolism*
Receptors, Progesterone / metabolism*
Reg. No./Substance:
0/Estrogen Receptor alpha; 0/RNA, Messenger; 0/Receptors, Estrogen; 0/Receptors, Progesterone

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