Document Detail


Oestrogen and progesterone receptor binding capacity and oestrogen receptor alpha expression (ERalpha mRNA) along the cervix of cycling ewes.
MedLine Citation:
PMID:  18402754     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of the present work was to study the oestrogen receptor (ER) and progesterone receptor (PR) binding capacity and the oestrogen receptor alpha (ERalpha) mRNA concentration in cranial and caudal cervix during the ovine oestrous cycle. Cervical samples of synchronised Corriedale ewes were obtained on Day 1 (n=7), 6 (n=6) or 13 (n=7) after oestrus detection (Day 0). The ER and PR binding capacity by ligand-binding assay and the ERalpha mRNA concentration by solution hybridisation in both cranial and caudal zones of the cervix were determined. The ER and PR binding capacity were higher (P<0.005) on Day 1 than on Days 6 and 13 in both cranial and caudal zones. The ERalpha mRNA concentrations were higher (P<0.0001) on Day 1 than on Days 6 and 13 only in the caudal zone. The PR binding capacity and ERalpha mRNA concentration were higher (P<0.005) in the caudal than in the cranial zone on Day 1. The ER and PR expression in the ovine cervix varied during the oestrous cycle in agreement with the known upregulation exerted by oestrogen and downregulation exerted by progesterone. Differences in ER and PR expression along the longitudinal axis of the ovine cervix were found, reflecting histological and functional differences between the cranial and caudal zones.
Authors:
M Rodríguez-Piñón; C Tasende; P Puime; E G Garófalo
Related Documents :
173044 - (3h)-estradiol binding by chick liver nuclear extracts: mechanism of increase in bindin...
19862954 - Profile and removal of endocrine disrupting chemicals by using an er/ar competitive lig...
588594 - High affinity estrogen binding by rabbit liver.
2611214 - Characterization and partial purification of an estrogen type ii binding site in chick ...
3607054 - Preferential binding of [3h]cholesteryl linoleyl ether-hdl3 by bovine adrenal membranes.
6274 - Removal of mn from spinach chloroplasts by sodium cyanide and the binding of mn2+ to mn...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction, fertility, and development     Volume:  20     ISSN:  1031-3613     ISO Abbreviation:  Reprod. Fertil. Dev.     Publication Date:  2008  
Date Detail:
Created Date:  2008-04-11     Completed Date:  2008-06-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8907465     Medline TA:  Reprod Fertil Dev     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  350-6     Citation Subset:  IM    
Affiliation:
Biochemistry, Department of Molecular and Cellular Biology, Faculty of Veterinary, Montevideo, Uruguay. marodri@adinet.com.uy
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cervix Uteri / metabolism*
Down-Regulation
Estrogen Receptor alpha / biosynthesis
Estrous Cycle / physiology*
Female
Gene Expression
RNA, Messenger / biosynthesis
Receptors, Estrogen / metabolism*
Receptors, Progesterone / metabolism*
Sheep
Up-Regulation
Chemical
Reg. No./Substance:
0/Estrogen Receptor alpha; 0/RNA, Messenger; 0/Receptors, Estrogen; 0/Receptors, Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Expression of mRNAs encoding oestrogen receptor (ER) alpha and ERbeta, androgen receptor and progest...
Next Document:  Homeodomain protein HLX is expressed primarily in cytotrophoblast cell types in the early pregnancy ...