Document Detail


Odorant feature detection: activity mapping of structure response relationships in the zebrafish olfactory bulb.
MedLine Citation:
PMID:  11606628     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The structural determinants of an odor molecule necessary and/or sufficient for interaction with the cognate olfactory receptor(s) are not known. Olfactory receptor neurons expressing the same olfactory receptor converge in the olfactory bulb. Thus, optical imaging of neuronal activity in the olfactory bulb can visualize at once the contributions by all the different olfactory receptors responsive to a particular odorant. We have used this technique to derive estimates about the structural requirements and minimal number of different zebrafish olfactory receptors that respond to a series of naturally occurring amino acids and some structurally related compounds. We report that the alpha-carboxyl group, the alpha-amino group, and l-conformation of the amino acid are all required for activation of amino acid-responsive receptors. Increasing carbon chain length recruits successively more receptors. With increasing concentrations, the activity patterns induced by a homolog series of amino acids became more similar to each other. At intermediate concentrations patterns were unique across substances and across concentrations. The introduction of a terminal amino group (charged) both recruits additional receptors and prevents binding to some of the receptors that were responsive to the unsubstituted analog. In contrast, the introduction of a beta-hydroxyl group (polar) excluded the odorants from some of the receptors that are capable of binding the unsubstituted analog. Cross-adaptation experiments independently confirmed these results. Thus, odorant detection requires several different receptors even for relatively simple odorants such as amino acids, and individual receptors require the presence of some molecular features, the absence of others, and tolerate still other molecular features.
Authors:
S H Fuss; S I Korsching
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  21     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-10-18     Completed Date:  2001-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8396-407     Citation Subset:  IM    
Affiliation:
Institut für Genetik, Universität zu Köln, 50674 Köln, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / chemistry,  metabolism,  pharmacology
Animals
Brain Mapping*
Calcium / metabolism
Dose-Response Relationship, Drug
Fluorescent Dyes
Hydroxy Acids / chemistry,  metabolism,  pharmacology
Male
Molecular Conformation
Olfactory Bulb / cytology,  drug effects,  physiology*
Olfactory Receptor Neurons / drug effects,  physiology*
Organic Chemicals
Presynaptic Terminals / metabolism
Receptors, Odorant / metabolism
Smell / physiology*
Stimulation, Chemical
Structure-Activity Relationship
Substrate Specificity
Zebrafish
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Fluorescent Dyes; 0/Hydroxy Acids; 0/Organic Chemicals; 0/Receptors, Odorant; 138067-55-7/calcium green; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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