| Odontoblast-targeted Bcl-2 overexpression impairs dentin formation. | |
| | |
MedLine Citation:
|
PMID: 20518070 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Apoptosis has been described extensively in tooth development, which is under tight control of multiple apoptosis regulators, including anti-apoptotic protein Bcl-2. However, it is totally unclear how Bcl-2 is related to odontogenesis, especially dentinogenesis. Using a transgenic mouse Col2.3Bcl-2 in which human Bcl-2 was overexpressed in odontoblasts, the effect of Bcl-2 on dentinogenesis was investigated. Overexpression of Bcl-2 was detected by immunohistochemistry and Western blot. Odontoblast apoptosis was evaluated by TUNEL and Western blot detection of cleaved caspase-3. Odontoblast differentiation was assessed by real-time PCR detection of dentin matrix expression. Dentin mineralization was evaluated by micro-CT in vivo, and alizarin red S staining and calcium content analysis in vitro. Bcl-2 was found to be overexpressed in odontoblasts and prevent their apoptosis. Odontoblast differentiation and mineralization was inhibited by Bcl-2, as evidenced by lower expressions of DMP-1, OC, and DSPP, and decreased odontoblast mineralization in vitro, as well as decreased dentin thickness and mineral density in vivo when compared to the wild-type animals. Inhibition of odontoblast differentiation by Bcl-2 occurs, at least partially, via a suppression of MEK-ERK1/2 signaling pathway. In conclusion, Bcl-2 overexpression prevents odontoblast apoptosis and impairs dentin formation, partially via an inhibition of odontoblast differentiation. This study revealed some novel functions of Bcl-2 in dentinogenesis in addition to its anti-apoptotic effect, which shed some light on the genetic complexity of tooth development. |
| | |
Authors:
|
Wenjian Zhang; Jun Ju; Gloria Gronowicz |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Journal of cellular biochemistry Volume: 111 ISSN: 1097-4644 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2010 Oct |
Date Detail:
|
Created Date: 2010-09-27 Completed Date: 2011-01-25 Revised Date: 2012-05-07 |
Medline Journal Info:
|
Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
|
Languages: eng Pagination: 425-32 Citation Subset: IM |
Copyright Information:
|
© 2010 Wiley-Liss, Inc. |
Affiliation:
|
Department of Diagnostic Sciences, University of Texas Dental Branch at Houston, Houston, Texas 77030, USA. wenjian.zhang@uth.tmc.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apoptosis / drug effects Calcification, Physiologic / drug effects Cell Differentiation / drug effects Dentinogenesis / drug effects* Drug Delivery Systems / methods Genes, bcl-2* Humans Mice Mice, Transgenic Odontoblasts / drug effects* Proto-Oncogene Proteins c-bcl-2 / administration & dosage*, genetics, pharmacology |
| Grant Support | |
ID/Acronym/Agency:
|
1R03DE019663-01A1/DE/NIDCR NIH HHS; R03 DE019663-01A1/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Proto-Oncogene Proteins c-bcl-2 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Mitotic drug targets.
Next Document: Proliferative arrest of neural cells induces prion protein synthesis, nanotube formation, and cell-t...