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Odontoblast-like MDPC-23 cells function as odontoclasts with the RANKL/M-CSF induction.
MedLine Citation:
PMID:  22770625     Owner:  NLM     Status:  Publisher    
The precursors of odontoclast and the molecular mechanism by which odontoclasts resorb dentine remain unclear. MDPC-23 cells were derived from dental papilla cells and possessed odontoblast characteristics such as expressing dentine sialophosphoprotein (Dspp) and dentine matrix acidic phosphoprotein 1 (Dmp1). Here we induced MDPC-23 cells to have odontoclast-like functions with receptor activator of nuclear factor-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). We found that tartrate-resistant acid phosphatase (TRAP) positive cells, TRAP-positive multinucleated cells on the dentine slices were significantly increased in RANKL/M-CSF induced cells. Osteoclast-specific genes such as Trap, osteopetrosis associated transmembrane protein 1 (Ostm1), chloride channel 7 (Clcn7), cathepsin K (Ctsk) as well as osteoclast specific transcription factor, microphthalmia transcription factor (MITF) were up-regulated in the treated cells, whilst the mRNA level of Dspp, Dmp1 and Opg were reduced in the induced cells. The intracellular environment became more acidic in RANKL/M-CSF treatment group which resulted in more absorptive pits in dentine slices. We suggested that RANKL/M-CSF might induce odontoblast-like MDPC-23 cells to differentiate into odontoclast-like cells or function as odontoclasts. Our data might provide a new explanation to the precursors of odontoclast and root resorption.
Xiaohong Duan; Ting Yang; Yanli Zhang; Xuan Wen; Yang Xue; Mi Zhou
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-4
Journal Detail:
Title:  Archives of oral biology     Volume:  -     ISSN:  1879-1506     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Department of Oral Biology, School of Stomatology, The Fourth Military Medical University, 145 Changle West Road, Xi'an, Shaanxi 710032, PR China.
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