Document Detail


Odd mating-type substances may work as precursor molecules of even mating-type substances in Paramecium caudatum.
MedLine Citation:
PMID:  11831778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mating-type substances are key molecules in the sexual recognition of the odd (O) and even (E) complementary mating-type cells in Paramecium caudatum. Indirect evidence suggested that the substances were proteins and were located on ventral surface cilia. Monoclonal antibodies inhibiting the mating reactivity of the O cells have been obtained. Using these antibodies, we tried to detect antigen molecules as dot-blot signals. Strong dot-blot signals of antigens were only detected from the mating reactive cells, but they were not detected from the well-fed and starved cells without mating reactivity. In addition to identifying the antigen on cilia and cytoplasm of the O cell, the antigen was detected from the cytoplasm of the E cells but never from their cilia. Furthermore, extracts of the E cells induced mating reaction with the living E cells but not with O cells. Thus, the O mating-type substances exist in the cytoplasm of the E mating-type cells, supporting strongly the hypothesis that O mating-type substances are precursor molecules of the E mating-type substances.
Authors:
X Xu; M Kumakura; E Kaku; M Takahashi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of eukaryotic microbiology     Volume:  48     ISSN:  1066-5234     ISO Abbreviation:  J. Eukaryot. Microbiol.     Publication Date:    2001 Nov-Dec
Date Detail:
Created Date:  2002-02-07     Completed Date:  2002-07-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9306405     Medline TA:  J Eukaryot Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  683-9     Citation Subset:  IM    
Affiliation:
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology
Antigens, Protozoan / genetics,  metabolism
Cilia / physiology
Conjugation, Genetic
Cytoplasm / physiology
Paramecium / genetics,  physiology*
Protein Precursors / genetics,  metabolism*
Protozoan Proteins / genetics,  metabolism*
Reproduction / genetics
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Protozoan; 0/Protein Precursors; 0/Protozoan Proteins

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