| Ocular tolerance to a topical formulation of hyaluronic acid and chitosan-based nanoparticles. | |
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MedLine Citation:
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PMID: 20335805 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Hyaluronic acid-chitosan nanoparticles (HA-CS NPs) have the potential to serve as a reliable drug delivery system to topically treat ocular surface disorders. We evaluated the in vivo uptake by ocular structures, the acute tolerance, and possible alterations of tear film physiology in rabbits. METHODS: Fluorescent HA-CS NPs (fl-HA-CS NPs) were prepared by ionotropic gelation using fluoresceinamine-labeled hyaluronic acid and resuspended in buffer. fl-HA-CS NPs (30 microL, 0.5 mg/mL) and fluoresceinamine-HA conjugate (30 microL) were instilled into rabbit eyes every 30 minutes for 6 hours. In vivo uptake and acute tolerance were characterized 24 hours after the first instillation and compared with preinstillation measurements. Clinical signs, including tear production, lacrimal drainage system patency and reflux, and ocular surface pathology, were evaluated. RESULTS: The rabbits showed no signs of ocular discomfort or irritation after exposure to HA-CS NPs. No macroscopic alteration in ocular surface structures was observed. fl-HA-CS NPs were present inside conjunctival and corneal epithelial cells, although the distribution within the cells was different. The fl-HA-CS NPs had no significant effects on tissue morphology and functionality, tear production, or drainage. CONCLUSION: Taken together, these data demonstrate that HA-CS NPs are a safe drug carrier for ocular surface application. |
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Authors:
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Laura Contreras-Ruiz; Mar?a de la Fuente; Carmen Garc?a-V?zquez; Victoria S?ez; Bego?a Seijo; Mar?a J Alonso; Margarita Calonge; Yolanda Diebold |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cornea Volume: 29 ISSN: 1536-4798 ISO Abbreviation: Cornea Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-26 Completed Date: 2010-06-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8216186 Medline TA: Cornea Country: United States |
Other Details:
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Languages: eng Pagination: 550-8 Citation Subset: IM |
Affiliation:
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Ocular Surface Group-IOBA, University of Valladolid, Valladolid, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chitosan / chemistry, pharmacokinetics, toxicity* Conjunctiva Cornea / drug effects* Drug Delivery Systems* Epithelial Cells / drug effects Female Fluorescamine Hyaluronic Acid / chemistry, pharmacokinetics, toxicity* Lacrimal Apparatus / drug effects Nanoparticles / chemistry, toxicity* Ophthalmic Solutions Rabbits Tears / secretion |
| Chemical | |
Reg. No./Substance:
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0/Ophthalmic Solutions; 38183-12-9/Fluorescamine; 9004-61-9/Hyaluronic Acid; 9012-76-4/Chitosan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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