Document Detail


Ocular MECP2 protein expression in patients with and without Rett syndrome.
MedLine Citation:
PMID:  20682201     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rett syndrome is a neurodevelopmental disorder caused by mutations in the methyl CpG binding protein 2 gene (MECP2). The MECP2 protein is expressed primarily in neurons, and mutations in the gene lead to the clinical features of Rett syndrome in human patients and neurologic deficits in murine models. Visual function is relatively preserved in Rett syndrome patients, but the cause is unknown. The eyes of two Rett syndrome patients who died of the disease were analyzed; no gross or microscopic changes were found. MECP2 expression was examined using immunohistochemistry; nuclear protein expression was largely limited to ganglion cells and the portion of the inner nuclear layer populated by amacrine cells. No significant differences in MECP2 protein level or distribution were identified in the two eyes from the Rett syndrome patients, compared with 11 controls. The findings were compared with MECP2 expression in the brain of these two subjects and in MECP2-deficient mice. The findings suggest that the normally limited expression of MECP2 in visual pathway neurons may underlie the intact vision observed in Rett syndrome.
Authors:
Deepali Jain; Kamaljeet Singh; Sankar Chirumamilla; Genila M Bibat; Mary E Blue; Sakkubai R Naidu; Charles G Eberhart
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Pediatric neurology     Volume:  43     ISSN:  1873-5150     ISO Abbreviation:  Pediatr. Neurol.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-08-04     Completed Date:  2010-11-12     Revised Date:  2011-08-01    
Medline Journal Info:
Nlm Unique ID:  8508183     Medline TA:  Pediatr Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  35-40     Citation Subset:  IM    
Affiliation:
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Animals
Brain / metabolism
Eye / metabolism*
Female
Humans
Immunohistochemistry
Infant
Infant, Newborn
Methyl-CpG-Binding Protein 2 / genetics,  metabolism*
Mice
Mice, Knockout
Middle Aged
Neurons / metabolism*
Rett Syndrome / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
HD24448/HD/NICHD NIH HHS; HHSN275200900011C//PHS HHS; P01 HD024448-20/HD/NICHD NIH HHS; UL1RR025005/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/MECP2 protein, human; 0/Methyl-CpG-Binding Protein 2
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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