Document Detail


Oct4-enhanced green fluorescent protein transgenic pigs: a new large animal model for reprogramming studies.
MedLine Citation:
PMID:  21126163     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The domesticated pig has emerged as an important tool for development of surgical techniques, advancement of xenotransplantation, creation of important disease models, and preclinical testing of novel cell therapies. However, germ line-competent pluripotent porcine stem cells have not yet been derived. This has been a major obstacle to genetic modification of pigs. The transcription factor Oct4 is essential for the maintenance of pluripotency and for reprogramming somatic cells to a pluripotent state. Here, we report the production of transgenic pigs carrying an 18 kb genomic sequence of the murine Oct4 gene fused to the enhanced green fluorescent protein (EGFP) cDNA (OG2 construct) to allow identification of pluripotent cells by monitoring Oct4 expression by EGFP fluorescence. Eleven viable transgenic piglets were produced by somatic cell nuclear transfer. Expression of the EGFP reporter construct was confined to germ line cells, the inner cell mass and trophectoderm of blastocysts, and testicular germ cells. Reprogramming of fibroblasts from these animals by fusion with pluripotent murine embryonic stem cells or viral transduction with human OCT4, SOX2, KLF4, and c-MYC cDNAs resulted in Oct4-EGFP reactivation. The OG2 pigs have thus proved useful for monitoring reprogramming and the induction and maintenance of pluripotency in porcine cells. In conclusion, the OG2 transgenic pigs are a new large animal model for studying the derivation and maintenance of pluripotent cells, and will be valuable for the development of cell therapy.
Authors:
Monika Nowak-Imialek; Wilfried A Kues; Bjoern Petersen; Andrea Lucas-Hahn; Doris Herrmann; Srividyameena Haridoss; Marianne Oropeza; Erika Lemme; Hans R Schöler; Joseph W Carnwath; Heiner Niemann
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Publication Detail:
Type:  Journal Article     Date:  2011-01-12
Journal Detail:
Title:  Stem cells and development     Volume:  20     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1563-75     Citation Subset:  IM    
Affiliation:
1 Institute of Farm Animal Genetics , Friedrich-Loeffler-Institut, Neustadt, Germany .
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