| Oct-1 transcription factor is a negative regulator of rat CYP1A1 expression via an octamer sequence in its negative regulatory element. | |
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MedLine Citation:
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PMID: 8632766 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The rat CYP1A1 negative regulatory element (NRE) contains AP-1 and Oct-1 motifs at -808 to -788 bp. The CYP1A1 sequence from -813 to -779 bp and an identical sequence bearing a point mutation in the octamer motif were synthesized. Gel mobility shift assays showed the formation of two complexes with the wild-type CYP1A1 sequence and nuclear extracts from H4IIE and HepG2 hepatoma cells and from rat liver. The formation of the major complex was significantly reduced with the mutant octamer-containing oligomer and was specifically competed by an Oct-1 oligodeoxyribonucleotide. The addition of Oct-1 antibody caused a supershift of the major complex. The presence of the wild-type sequence, but not the mutant octamer sequence, caused a 3-fold decrease in SV40 enhancerless promoter activity in transfected HepG2 cells. Co-transfection of an Oct-1 expression vector with rat CYP1A1 NRE octamer-containing, promoter/reporter gene constructs specifically further decreased promoter activity of the wild-type octamer-containing constructs in HepG2 cells. The results indicate that Oct-1 binds to the rat CYP1A1 promoter NRE and is a negative regulator of rat CYP1A1 expression. |
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Authors:
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K Sterling; E Bresnick |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular pharmacology Volume: 49 ISSN: 0026-895X ISO Abbreviation: Mol. Pharmacol. Publication Date: 1996 Feb |
Date Detail:
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Created Date: 1996-07-03 Completed Date: 1996-07-03 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0035623 Medline TA: Mol Pharmacol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 329-37 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Molecular Toxicology, University of Massachusetts Medical Center, Worcester 01655, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Binding Sites Carcinoma, Hepatocellular Cell Line Cell Nucleus / metabolism Cytochrome P-450 Enzyme System / biosynthesis*, genetics* DNA-Binding Proteins* Gene Expression Regulation, Enzymologic* Homeodomain Proteins / biosynthesis, metabolism* Host Cell Factor C1 Humans Kinetics Liver Neoplasms Luciferases / biosynthesis, metabolism Molecular Sequence Data Mutagenesis, Site-Directed Octamer Transcription Factor-1 Oligodeoxyribonucleotides Point Mutation Promoter Regions, Genetic Rats Recombinant Proteins / biosynthesis, metabolism Regulatory Sequences, Nucleic Acid* Transcription Factors / biosynthesis, metabolism* Transfection |
| Grant Support | |
ID/Acronym/Agency:
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CA36106/CA/NCI NIH HHS; ES03980/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/HCFC1 protein, human; 0/Homeodomain Proteins; 0/Host Cell Factor C1; 0/Octamer Transcription Factor-1; 0/Oligodeoxyribonucleotides; 0/POU2F1 protein, human; 0/Pou2f1 protein, rat; 0/Recombinant Proteins; 0/Transcription Factors; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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