Document Detail


Occurrence of urolithins, gut microbiota ellagic acid metabolites and proliferation markers expression response in the human prostate gland upon consumption of walnuts and pomegranate juice.
MedLine Citation:
PMID:  19885850     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Epidemiology supports the important role of nutrition in prostate cancer (PCa) prevention. Pomegranate juice (PJ) exerts protective effects against PCa, mainly attributed to PJ ellagitannins (ETs). Our aim was to assess whether ETs or their metabolites ellagic acid and urolithins reach the human prostate upon consumption of ET-rich foods and to evaluate the effect on the expression of three proliferation biomarkers. Sixty-three patients with BPH or PCa were divided into controls and consumers of walnuts (35 g walnuts/day) or pomegranate (200 mL PJ/day) for 3 days before surgery. Independently of the ETs source, the main metabolite detected was urolithin A glucuronide, (3,8-dihydroxy-6H-dibenzo[b,d]pyran-6-one glucuronide) (up to 2 ng/g) together with the traces of urolithin B glucuronide, (3-hydroxy-6H-dibenzo[b,d]pyran-6-one glucuronide) and dimethyl ellagic acid. The small number of prostates containing metabolites was likely caused by clearance of the compounds during the fasting. This was corroborated in a parallel rat study and thus the presence of higher quantities of metabolites at earlier time points cannot be discarded. No apparent changes in the expression of CDKN1A, MKi-67 or c-Myc were found after consumption of the walnuts or PJ. Our results suggest that urolithin glucuronides and dimethyl ellagic acid may be the molecules responsible for the beneficial effects of PJ against PCa.
Authors:
Antonio González-Sarrías; Juan A Giménez-Bastida; María T García-Conesa; María B Gómez-Sánchez; Noelia V García-Talavera; Angel Gil-Izquierdo; Carmen Sánchez-Alvarez; Luis O Fontana-Compiano; Juan P Morga-Egea; Francisco A Pastor-Quirante; Francisco Martínez-Díaz; Francisco A Tomás-Barberán; Juan Carlos Espín
Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular nutrition & food research     Volume:  54     ISSN:  1613-4133     ISO Abbreviation:  Mol Nutr Food Res     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-15     Completed Date:  2010-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101231818     Medline TA:  Mol Nutr Food Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  311-22     Citation Subset:  IM    
Affiliation:
Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS-CSIC, Murcia, Spain.
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MeSH Terms
Descriptor/Qualifier:
Aged
Animals
Beverages / analysis
Coumarins / administration & dosage,  chemistry,  metabolism*,  urine
Cyclin-Dependent Kinase Inhibitor p21 / genetics,  metabolism
Ellagic Acid / chemistry,  metabolism*,  urine
Fruit* / chemistry
Gene Expression Regulation, Neoplastic
Humans
Hydrolyzable Tannins / administration & dosage,  chemistry,  metabolism
Intestines / microbiology
Intracellular Signaling Peptides and Proteins / genetics,  metabolism
Juglans* / chemistry
Male
Middle Aged
Nuclear Proteins / genetics,  metabolism
Plant Extracts / administration & dosage,  chemistry
Prostate / metabolism*
Prostatic Hyperplasia / metabolism,  surgery,  urine
Prostatic Neoplasms / metabolism*,  prevention & control,  surgery,  urine
Proto-Oncogene Proteins c-myc / genetics,  metabolism
Punicaceae* / chemistry
RNA, Messenger / metabolism
Rats
Rats, Sprague-Dawley
Tumor Markers, Biological / metabolism
Chemical
Reg. No./Substance:
0/CDKN1A protein, human; 0/Coumarins; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Hydrolyzable Tannins; 0/Intracellular Signaling Peptides and Proteins; 0/MKI67IP protein, human; 0/MYC protein, human; 0/Nuclear Proteins; 0/Plant Extracts; 0/Proto-Oncogene Proteins c-myc; 0/RNA, Messenger; 0/Tumor Markers, Biological; 1143-70-0/3,8-dihydroxy-6H-dibenzo(b,d)pyran-6-one; 476-66-4/Ellagic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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