Document Detail


Occurrence of the tumor-specific, calcium-binding protein, oncomodulin, in virally transformed normal rat kidney cells.
MedLine Citation:
PMID:  6311406     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oncomodulin, an apparently tumor-specific calcium-binding protein, has been detected in many chemically induced rat hepatomas. It is now possible to detect, by radioimmunoassay and immunofluorescence, the presence of oncomodulin in normal rat kidney cells virally transformed by avian sarcoma virus. By contrast, it was not detected in uninfected, nonneoplastic normal rat kidney cells. The protein was isolated and purified by a novel high-performance liquid chromatography procedure and shown to be identical to that isolated previously from rat hepatoma. The cellular levels of oncomodulin approached the levels of calmodulin in avian sarcoma virus-transformed normal rat kidney cells, suggesting that the total calcium-binding activity of the cell may play a role in expression of the transformed phenotype.
Authors:
J P Durkin; L M Brewer; J P MacManus
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  43     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1983 Nov 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5390-4     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Animals
Avian Sarcoma Viruses / genetics*
Calcium-Binding Proteins / genetics*,  isolation & purification
Cell Transformation, Viral*
Chromatography, High Pressure Liquid
Fluorescent Antibody Technique
Kidney Neoplasms / analysis
Liver Neoplasms, Experimental / analysis
Neoplasm Proteins / genetics*,  isolation & purification
Radioimmunoassay
Rats
Grant Support
ID/Acronym/Agency:
X-ROI-CA-31898/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Calcium-Binding Proteins; 0/Neoplasm Proteins; 0/oncomodulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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