Document Detail

Occurrence of oxidized metabolites of arachidonic acid esterified to phospholipids in murine lung tissue.
MedLine Citation:
PMID:  9735144     Owner:  NLM     Status:  MEDLINE    
Isolation and characterization of murine pulmonary phospholipids revealed the normal occurrence of 10 isobaric eicosanoids corresponding to the incorporation of one oxygen atom into the arachidonate esterified to glycerophospholipids. Lungs from mice were removed and lipids were extracted and then separated into free carboxylic acid and phospholipids. Phospholipids were hydrolyzed to yield the free carboxylic acids prior to analysis. Reverse-phase HPLC and electrospray tandem mass spectrometry were used to identify and quantitate six monohydroxyeicosatetraenoic (HETE) and four epoxyeicosatetraenoic (EET) acid regioisomers using d8-HETE as internal standard. HETEs esterified to phospholipids were found to increase following intratracheal administration of tBuOOH (36 mg/kg), but not the levels of esterified EETs. Chiral analysis of esterified 15-HETE revealed an R/S ratio of 0.96, suggesting operation of a free radical mechanism responsible for generation of this monohydroxy arachidonate phospholipid, and this enantiomeric ratio was 1.10 following treatment of the mouse lung with tBuOOH. These results are consistent with a free-radical-based mechanism of oxidation of pulmonary glycerophospholipids containing arachidonate.
T Nakamura; P M Henson; R C Murphy
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  262     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-10-22     Completed Date:  1998-10-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  23-32     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Academic Press.
National Jewish Medical and Research Center, 1400 Jackson Street, Denver, Colorado, 80206, USA.
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MeSH Terms
Arachidonic Acid / metabolism*
Chromatography, High Pressure Liquid
Eicosanoids / analysis*
Esters / analysis*
Lipoxygenase / metabolism
Lung / metabolism*
Mass Spectrometry
Mice, Inbred Strains
Oxygen / metabolism
Peroxides / metabolism
Phosphatidic Acids / analysis
Prostaglandin-Endoperoxide Synthases / metabolism
Grant Support
Reg. No./Substance:
0/Eicosanoids; 0/Esters; 0/Peroxides; 0/Phosphatidic Acids; 506-32-1/Arachidonic Acid; 75-91-2/tert-Butylhydroperoxide; 7782-44-7/Oxygen; EC; EC Synthases

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