| Occurrence of oxidized metabolites of arachidonic acid esterified to phospholipids in murine lung tissue. | |
| | |
MedLine Citation:
|
PMID: 9735144 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Isolation and characterization of murine pulmonary phospholipids revealed the normal occurrence of 10 isobaric eicosanoids corresponding to the incorporation of one oxygen atom into the arachidonate esterified to glycerophospholipids. Lungs from mice were removed and lipids were extracted and then separated into free carboxylic acid and phospholipids. Phospholipids were hydrolyzed to yield the free carboxylic acids prior to analysis. Reverse-phase HPLC and electrospray tandem mass spectrometry were used to identify and quantitate six monohydroxyeicosatetraenoic (HETE) and four epoxyeicosatetraenoic (EET) acid regioisomers using d8-HETE as internal standard. HETEs esterified to phospholipids were found to increase following intratracheal administration of tBuOOH (36 mg/kg), but not the levels of esterified EETs. Chiral analysis of esterified 15-HETE revealed an R/S ratio of 0.96, suggesting operation of a free radical mechanism responsible for generation of this monohydroxy arachidonate phospholipid, and this enantiomeric ratio was 1.10 following treatment of the mouse lung with tBuOOH. These results are consistent with a free-radical-based mechanism of oxidation of pulmonary glycerophospholipids containing arachidonate. |
| | |
Authors:
|
T Nakamura; P M Henson; R C Murphy |
Related Documents
:
|
12669824 - Phospholipid fa from indian ocean tunicates eudistoma bituminis and cystodytes violatin... 400824 - Target substances of some antifungal agents in the cell membrane. 990304 - Modification of the ehrlich ascites tumor cell nuclear lipids. 6308684 - Increased arachidonic acid content in platelet phospholipids from diabetic patients. 15621204 - P66shca and ageing: modulation by longevity-promoting agent aurintricarboxylic acid. 1359904 - Changes in neurotransmitter levels associated with the deficiency of some essential ami... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Analytical biochemistry Volume: 262 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1998 Aug |
Date Detail:
|
Created Date: 1998-10-22 Completed Date: 1998-10-22 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 23-32 Citation Subset: IM |
Copyright Information:
|
Copyright 1998 Academic Press. |
Affiliation:
|
National Jewish Medical and Research Center, 1400 Jackson Street, Denver, Colorado, 80206, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Arachidonic Acid / metabolism* Chromatography, High Pressure Liquid Eicosanoids / analysis* Esters / analysis* Isomerism Lipoxygenase / metabolism Lung / metabolism* Mass Spectrometry Mice Mice, Inbred Strains Oxidation-Reduction Oxygen / metabolism Peroxides / metabolism Phosphatidic Acids / analysis Prostaglandin-Endoperoxide Synthases / metabolism tert-Butylhydroperoxide |
| Grant Support | |
ID/Acronym/Agency:
|
HL34303/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Eicosanoids; 0/Esters; 0/Peroxides; 0/Phosphatidic Acids; 506-32-1/Arachidonic Acid; 75-91-2/tert-Butylhydroperoxide; 7782-44-7/Oxygen; EC 1.13.11.12/Lipoxygenase; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Short-term insulin-induced glycogen formation in primary hepatocytes as a screening bioassay for ins...
Next Document: Determination of ATP and its metabolites released from rat caudal artery by isocratic ion-pair rever...