Document Detail


Occurrence of natural triploidy in Rhamdia quelen (Siluriformes, Heptapteridae).
MedLine Citation:
PMID:  20882489     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Five specimens of Rhamdia quelen collected from the Lindóia Stream, PR, Brazil, were cytogenetically analyzed. The diploid chromosome number found was 58, including 30 metacentric, 16 submetacentric, 10 subtelocentric, and 2 acrocentric chromosomes. Supernumerary or B chromosomes, frequently observed in this fish group, were not detected. One of the individuals was triploid, with 3n = 87. A silver-stained nucleolar organizer region was found on a pair of submetacentric chromosomes of the diploid specimens, and on three chromosomes of the triploid individual, confirming triploidy. Treatment with fluorochrome chromomycin A(3) revealed fluorescent bands coincident with those of the silver-stained nucleolar organizer region, in both diploid and triploid individuals, showing that this is a GC-rich region. Heterochromatin distribution was visualized by the C-banding technique, mainly in the terminal chromosome regions of the individuals and was also observed in the pericentromeric regions of some chromosomes and at both telomeres.
Authors:
J R Tsuda; V P O de Moraes; L Giuliano-Caetano; A L Dias
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-28
Journal Detail:
Title:  Genetics and molecular research : GMR     Volume:  9     ISSN:  1676-5680     ISO Abbreviation:  Genet. Mol. Res.     Publication Date:  2010  
Date Detail:
Created Date:  2010-09-30     Completed Date:  2010-11-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101169387     Medline TA:  Genet Mol Res     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  1929-35     Citation Subset:  IM    
Affiliation:
Departamento de Biologia Geral, CCB, Universidade Estadual de Londrina, Londrina, PR, Brasil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brazil
Catfishes / genetics*
Cytogenetics / methods
Polyploidy*

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