Document Detail

Occurrence and molecular characterization of fusidic acid resistance mechanisms among Staphylococcus spp. from European countries (2008).
MedLine Citation:
PMID:  20430787     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: To determine fusidic acid resistance rates (MICs of > or = 2 mg/L) and the prevalence of fusidic acid resistance mechanisms among Staphylococcus spp. collected from European countries (2008). METHODS: Staphylococcal isolates (3134) collected from Europe were tested by CLSI broth microdilution. Isolates displaying a fusidic acid MIC of > or = 2 mg/L (non-susceptible; European Committee on Antimicrobial Susceptibility Testing breakpoint) were tested for fusB, fusC and fusD. The fusidic acid target sites fusA and fusE were then sequenced, and a method for the detection of the fusA mutation L461K was developed. Selected isolates were typed by PFGE. RESULTS: Fusidic acid resistance rates were higher among coagulase-negative staphylococci (CoNS) compared with Staphylococcus aureus. Acquired fusidic acid resistance genes were detected in 64.9% of the samples; fusB and fusC were detected among 10.1% and 16.9% of the fusidic acid-resistant S. aureus and among 26.5% and 11.3% of the CoNS, respectively. Ireland and Greece showed the highest S. aureus fusidic acid resistance levels, with low rates of acquired fusidic acid resistance genes. Isolates from these countries displayed MIC values of > or = 512 mg/L, the presence of the elongation factor G L461K alteration and clonal occurrences. Low S. aureus fusidic acid resistance rates (1%-3%) were observed in Germany, Israel, Italy, Poland, Spain and Sweden. Isolates with MIC values < or = 64 mg/L showed a great diversity of acquired fusidic acid resistance mechanisms. Acquired fusidic acid resistance genes were detected in the majority of fusidic acid-resistant isolates from Belgium, France, Switzerland, Sweden, Italy and the UK (72.2%-92.9%), and were slightly less frequent in Germany, Spain and Israel (61.3%-66.7%). CONCLUSIONS: This contemporary study showed that acquired fusidic acid resistance genes were prevalent among fusidic acid-non-susceptible European staphylococcal isolates.
Mariana Castanheira; Amy A Watters; Rodrigo E Mendes; David J Farrell; Ronald N Jones
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-29
Journal Detail:
Title:  The Journal of antimicrobial chemotherapy     Volume:  65     ISSN:  1460-2091     ISO Abbreviation:  J. Antimicrob. Chemother.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-10     Completed Date:  2010-09-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7513617     Medline TA:  J Antimicrob Chemother     Country:  England    
Other Details:
Languages:  eng     Pagination:  1353-8     Citation Subset:  IM    
JMI Laboratories, North Liberty, IA 52317, USA.
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MeSH Terms
Anti-Bacterial Agents / pharmacology*
Bacterial Proteins / genetics
Bacterial Typing Techniques
Base Sequence
DNA Fingerprinting / methods
DNA, Bacterial / chemistry,  genetics
Drug Resistance, Bacterial*
Electrophoresis, Gel, Pulsed-Field
Fusidic Acid / pharmacology*
Genes, Bacterial*
Microbial Sensitivity Tests
Molecular Sequence Data
Mutation, Missense
Peptide Elongation Factor G / genetics
Sequence Analysis, DNA
Staphylococcal Infections / microbiology*
Staphylococcus / classification,  drug effects*,  genetics*,  isolation & purification
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/DNA, Bacterial; 0/FusA protein, Staphylococcus aureus; 0/FusB protein, Staphylococcus aureus; 0/Peptide Elongation Factor G; 6990-06-3/Fusidic Acid

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