Document Detail

Occurrence of immunoreactivity for adipocyte-type fatty acid binding protein in degenerating granulosa cells in atretic antral follicles of mouse ovary.
MedLine Citation:
PMID:  16733794     Owner:  NLM     Status:  MEDLINE    
The localization of adipocyte-type fatty acid binding protein (A-FABP) in the mature mouse ovary was examined by immuno-light and electron microscopy. Solitary round cells showing the distinct immunoreactivity for A-FABP were detected in 1-6 antral follicles. In sets of two consecutive sections in a mirror alignment on slide glasses which were treated for immunoreactivity for A-FABP and TUNEL reaction separately, cells immunoreactive for A-FABP appeared in the same antral follicles as containing cells exhibiting TUNEL-reaction. In immunoelectron microscopy, A-FABP-immunopositive cells were found to contain highly electron-dense nuclei of round, irregular or crescent shapes together with cytoplasmic remnants without any features of macrophages or cells of extrinsic origin. Therefore the cells were identified as apoptotic granulosa cells. The apoptotic cells immunoreactive for A-FABP were often seen to be enclosed/engulfed in adjacent cells exhibiting normal ultrastructures without containing numerous lysosomes. The present findings suggest that A-FABP is involved in the apoptosis of ovarian granulosa cells, probably through its interaction with peroxisome proliferator activated receptors.
Mohammad Reza Nourani; Yuji Owada; Noriko Kitanaka; Hiroyuki Sakagami; Hisae Hoshi; Hiroo Iwasa; Friedrich Spener; Hisatake Kondo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-04-28
Journal Detail:
Title:  Journal of molecular histology     Volume:  36     ISSN:  1567-2379     ISO Abbreviation:  J. Mol. Histol.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2006-05-30     Completed Date:  2007-04-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101193653     Medline TA:  J Mol Histol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  491-7     Citation Subset:  IM    
Division of Histology, Department of Cell Biology, Graduate School of Medicine, Tohoku University, Sendai, Japan.
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MeSH Terms
Adipocytes / metabolism*
Fatty Acid-Binding Proteins / immunology*
Granulosa Cells / immunology*,  pathology*,  ultrastructure
In Situ Nick-End Labeling
Mice, Inbred C57BL
Reg. No./Substance:
0/Fabp4 protein, mouse; 0/Fatty Acid-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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