Document Detail

Occurrence and distribution of atrial natriuretic peptide-containing cells in the left ventricle of hypertensive rats. Effect of antihypertensive treatment.
MedLine Citation:
PMID:  11236005     Owner:  NLM     Status:  MEDLINE    
In the ventricles of adult mammalian hearts, production of atrial natriuretic peptide (ANP) is negligible, restricted to the impulse-conducting cells, the papillary muscles, and a minority of subendocardial myocytes. ANP expression is reinduced in the ventricles of pressure-overloaded and failing hearts and is frequently used as a marker for myocyte hypertrophy. Using an immunohistochemical approach, we have characterized the size distribution of ANP-containing myocytes in the left ventricle of the spontaneously hypertensive rat (SHR) before and after chronic antihypertensive therapy and compared the results to age-matched normotensive Wistar rats (WR). Our findings show that in SHR the frequency of cells presenting ANP granularity is positively correlated with myocyte size (r=0.746, P<0.02). The highest proportion of ANP-positive myocytes (55-57%) was measured among cells of diameters 30-34 microm. In any corresponding cell size, the proportion of ANP-presenting myocytes was five- to tenfold higher in SHR than in the normotensive WR. We studied the effects of the antihypertensive drugs captopril, hydralazine, and nifedipine and found that, regardless of their effect on blood pressure or hypertrophy, all three eliminated ANP immunoproducts from the majority of the left ventricular myocytes and reduced the level of ANP mRNA, captopril being the most effective. The positive correlation between myocyte size and ANP expression was not maintained in the hearts of drug-treated SHR. Myocytes on the border of fibrotic areas or in regions of ANP presentation within the normal heart resisted the suppressive effect of the antihypertensive therapy, indicating that blood pressure or hypertrophy are not the sole correlates for ANP expression.
E Kaganovsky; V Belkin; Y Barhum; J Schaper; W Schaper; G Kessler-Icekson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell and tissue research     Volume:  303     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-03-08     Completed Date:  2001-03-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  57-67     Citation Subset:  IM    
The Basil and Gerald Felsenstein Medical Research Center, Rabin Medical Center, Sackler School of Medicine, Tel-Aviv University, Israel.
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MeSH Terms
Antihypertensive Agents / pharmacology*
Atrial Natriuretic Factor / analysis*,  genetics*
Blood Pressure / physiology
Blotting, Northern
Captopril / pharmacology*
Cell Size / drug effects,  physiology
Gene Expression / drug effects,  physiology
Heart Ventricles / chemistry,  cytology
Hydralazine / pharmacology
Hypertension / drug therapy*,  physiopathology
Muscle Fibers, Skeletal / chemistry,  cytology
Myocardium / chemistry*,  cytology
Nifedipine / pharmacology
Organ Size
RNA, Messenger / analysis
Rats, Inbred SHR
Rats, Wistar
Vasodilator Agents / pharmacology
Ventricular Function
Reg. No./Substance:
0/Antihypertensive Agents; 0/RNA, Messenger; 0/Vasodilator Agents; 21829-25-4/Nifedipine; 62571-86-2/Captopril; 85637-73-6/Atrial Natriuretic Factor; 86-54-4/Hydralazine

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