| Occurrence of aflatoxins in selected processed foods from pakistan. | |
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MedLine Citation:
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PMID: 22942705 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A total of 125 (ready to eat) processed food samples (70 intended for infant and 55 for adult intake) belonging to 20 different food categories were analyzed for aflatoxins contamination using Reverse Phase High Performance Liquid Chromatography (RP-HPLC) with fluorescent detection. A solvent mixture of acetonitrile-water was used for the extraction followed by immunoaffinity clean-up to enhance sensitivity of the method. The limit of detection (LOD) (0.01-0.02 ng·g(-1)) and limit of quantification (LOQ) (0.02 ng·g(-1)) was established for aflatoxins based on signal to noise ratio of 3:1 and 10:1, respectively. Of the processed food samples tested, 38% were contaminated with four types of aflatoxins, i.e., AFB1 (0.02-1.24 μg·kg(-1)), AFB2 (0.02-0.37 μg·kg(-1)), AFG1 (0.25-2.7 μg·kg(-1)) and AFG2 (0.21-1.3 μg·kg(-1)). In addition, the results showed that 21% of the processed foods intended for infants contained AFB1 levels higher than the European Union permissible limits (0.1 μg·kg(-1)), while all of those intended for adult consumption had aflatoxin contamination levels within the permitted limits. |
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Authors:
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Muhammad Mushtaq; Bushra Sultana; Farooq Anwar; Muhammad Zargham Khan; Muhammad Ashrafuzzaman |
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Publication Detail:
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Type: Journal Article Date: 2012-07-04 |
Journal Detail:
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Title: International journal of molecular sciences Volume: 13 ISSN: 1422-0067 ISO Abbreviation: Int J Mol Sci Publication Date: 2012 |
Date Detail:
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Created Date: 2012-09-03 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101092791 Medline TA: Int J Mol Sci Country: Switzerland |
Other Details:
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Languages: eng Pagination: 8324-37 Citation Subset: - |
Affiliation:
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Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad 38040, Pakistan; E-Mail: mmushtaq_doc@yahoo.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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