Document Detail


Occurrence of D-Amino Acids and a pyridoxal 5'-phosphate-dependent aspartate racemase in the acidothermophilic archaeon, Thermoplasma acidophilum.
MedLine Citation:
PMID:  11181048     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Free D-amino acid content in some archaea was investigated and D-forms of several amino acids were found in them. In the acidothermophilic archaeon, Thermoplasma acidophilum, the proportion of D-aspartate (D-Asp) to total Asp was as high as 39.7%. Crude extracts of Thermoplasma acidophilum had Asp-specific racemase activity that was pyridoxal 5'-phosphate (PLP)-dependent. The relative insensitivity to a SH-modifying reagent distinguished this activity from those of the PLP-independent Asp racemases found in other hyperthermophilic archaea (Matsumoto, M., et al., J. Bacteriol. 181, 6560-6563 1999). Thus, high levels of d-Asp should be produced by a new type(s) of Asp-specific racemase in Thermoplasma acidophilum, although the function of d-Asp in this archaeon remains unknown.
Authors:
Z Long; J A Lee; T Okamoto; M Sekine; N Nimura; K Imai; M Yohda; T Maruyama; M Sumi; N Kamo; A Yamagishi; T Oshima; H Homma
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  281     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-03-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  317-21     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Affiliation:
School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alanine / chemistry,  metabolism
Amino Acid Isomerases / antagonists & inhibitors,  metabolism*
Amino Acids / chemistry,  metabolism*
Aspartic Acid / chemistry,  metabolism
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology
Ethylmaleimide / pharmacology
Glutamic Acid / chemistry,  metabolism
Leucine / chemistry,  metabolism
Lysine / chemistry,  metabolism
Phenylalanine / chemistry,  metabolism
Pyridoxal Phosphate / physiology
Stereoisomerism
Thermoplasma / enzymology,  metabolism*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Enzyme Inhibitors; 128-53-0/Ethylmaleimide; 54-47-7/Pyridoxal Phosphate; 56-41-7/Alanine; 56-84-8/Aspartic Acid; 56-86-0/Glutamic Acid; 56-87-1/Lysine; 61-90-5/Leucine; 63-91-2/Phenylalanine; EC 5.1.1.-/Amino Acid Isomerases; EC 5.1.1.13/aspartate racemase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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