Document Detail

Occurrence of the Crabtree effect in HeLa cells.
MedLine Citation:
PMID:  9636997     Owner:  NLM     Status:  MEDLINE    
The occurrence of a Crabtree effect in HeLa cells was detected. Some properties of pyruvate kinase (PK) were also evaluated. Hexose phosphate, triose-phosphate and phosphoenolpyruvate (PEP) significantly decreased the oxygen consumption of digitonin-permeabilized HeLa cells, which were oxidizing succinate. The Crabtree effect promoted by PEP was concentration-dependent and was lowered by an increase of ADP concentration, suggesting a participation of PK. The dependence of fructose-1,6-bisphosphate (FDP) by HeLa cell PK was observed. The PK of HeLa cells was inhibited by L-alanine only in the absence of FDP, while in the presence of the metabolite, an increase in the activity was observed. PK was also inhibited in the presence of L-histidine and L-leucine, while L-serine promoted activation. L-Cysteine and L-phenylalanine also inhibited the PK of HeLa cells. This, together with the sigmoidal character in relation to substrate concentration, suggests the presence of the K-type of PK in HeLa cells.
R F Melo; F R Stevan; A P Campello; E G Carnieri; M B de Oliveira
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  16     ISSN:  0263-6484     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-07-20     Completed Date:  1998-07-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  99-105     Citation Subset:  IM    
Departamento do Bioquímica da Universidade Federal do Paraná Curitiba, Brasil.
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MeSH Terms
Cell Division / physiology
Cell Respiration / physiology
Cell-Free System
Glucose / metabolism*
Glycolysis / physiology
Hela Cells
Pyruvate Kinase / metabolism
Reg. No./Substance:
50-99-7/Glucose; EC Kinase

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