| Occurrence of the Crabtree effect in HeLa cells. | |
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MedLine Citation:
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PMID: 9636997 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The occurrence of a Crabtree effect in HeLa cells was detected. Some properties of pyruvate kinase (PK) were also evaluated. Hexose phosphate, triose-phosphate and phosphoenolpyruvate (PEP) significantly decreased the oxygen consumption of digitonin-permeabilized HeLa cells, which were oxidizing succinate. The Crabtree effect promoted by PEP was concentration-dependent and was lowered by an increase of ADP concentration, suggesting a participation of PK. The dependence of fructose-1,6-bisphosphate (FDP) by HeLa cell PK was observed. The PK of HeLa cells was inhibited by L-alanine only in the absence of FDP, while in the presence of the metabolite, an increase in the activity was observed. PK was also inhibited in the presence of L-histidine and L-leucine, while L-serine promoted activation. L-Cysteine and L-phenylalanine also inhibited the PK of HeLa cells. This, together with the sigmoidal character in relation to substrate concentration, suggests the presence of the K-type of PK in HeLa cells. |
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Authors:
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R F Melo; F R Stevan; A P Campello; E G Carnieri; M B de Oliveira |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell biochemistry and function Volume: 16 ISSN: 0263-6484 ISO Abbreviation: Cell Biochem. Funct. Publication Date: 1998 Jun |
Date Detail:
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Created Date: 1998-07-20 Completed Date: 1998-07-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8305874 Medline TA: Cell Biochem Funct Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 99-105 Citation Subset: IM |
Affiliation:
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Departamento do Bioquímica da Universidade Federal do Paraná Curitiba, Brasil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Division
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physiology Cell Respiration / physiology Cell-Free System Glucose / metabolism* Glycolysis / physiology Hela Cells Humans Pyruvate Kinase / metabolism |
| Chemical | |
Reg. No./Substance:
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50-99-7/Glucose; EC 2.7.1.40/Pyruvate Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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