| Occupational rhinitis and bronchial asthma due to artichoke (Cynara scolymus). | |
| | |
MedLine Citation:
|
PMID: 12877457 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: The artichoke is a perennial horticultural plant that belongs to the Compositae family. OBJECTIVE: To present case studies of 2 vegetable warehouse workers who developed occupational rhinitis and bronchial asthma by sensitization to artichoke. METHODS: Skin prick tests with common inhalants and foods were performed. Specific IgE to artichoke, Parietaria judaica pollen, and Olea europaea pollen extracts was measured by a specific IgE enzyme immunosorbent assay kit. Molecular mass of the allergens was studied by the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) immunoblotting technique. Patients underwent a nasal challenge test, and one patient provided peak expiratory flow rate (PEFR) measurements in her workplace. RESULTS: In both patients, results of skin prick tests to artichoke were positive. Levels of specific IgE for artichoke were 0.68 kU/L in patient 1 and 2.14 kU/L in patient 2. The protein composition of the artichoke extract, studied by SDS-PAGE, showed that most bands ranged from 30 to 14 kDa. The IgE-binding bands with the serum samples of patient 1 showed apparent molecular masses of 56, 48, 38, 31, 27, 25, 16, and 15 kDa; however, the serum samples of patient 2 showed IgE bands of 21 and 19 kDa. Western blotting of artichoke extract showed a complete inhibition of IgE-binding bands when serum samples were preincubated with P. judaica pollen extract. Nasal challenge with artichoke extract triggered a peak nasal inspiratory flow decrease of 81% and 85% in patient 1 and patient 2, respectively. Finally, patient 1 recorded a PEFR decrease of up to 36% after exposure to artichoke in her workplace. CONCLUSIONS: SDS-PAGE immunoblotting inhibition performed for the artichoke extract showed a total disappearance of the specific IgE binding bands when serum samples were previously incubated with P. judaica pollen extract, thus establishing the existence of a serologic cross-reactivity between artichoke and P. judaica pollen. |
| | |
Authors:
|
Juan-Carlos Miralles; Javier García-Sells; Borja Bartolomé; José-María Negro |
Related Documents
:
|
7217397 - Studies of phenylketonurics with dermatitis. 3972147 - Lymphocyte transformation test in patients with american leishmaniasis. 21146727 - Pruritus in the elderly. 12847497 - The natural progression of peanut allergy: resolution and the possibility of recurrence. 23252617 - Replaced platelet concentrates containing a new additive solution, m-sol: safety and ef... 15511477 - Long-term results of thoracoscopic sympathectomy for hyperhidrosis. |
Publication Detail:
|
Type: Case Reports; Journal Article |
Journal Detail:
|
Title: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology Volume: 91 ISSN: 1081-1206 ISO Abbreviation: Ann. Allergy Asthma Immunol. Publication Date: 2003 Jul |
Date Detail:
|
Created Date: 2003-07-24 Completed Date: 2003-08-14 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 9503580 Medline TA: Ann Allergy Asthma Immunol Country: United States |
Other Details:
|
Languages: eng Pagination: 92-5 Citation Subset: IM |
Affiliation:
|
University General Hospital, Murcia, Spain. jmirallesl@meditex.es |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Allergens / immunology Asthma / immunology* Cynara scolymus / immunology* Humans Hypersensitivity, Immediate / immunology Immunoglobulin E / blood Middle Aged Occupational Diseases / immunology* Occupational Exposure / adverse effects Peak Expiratory Flow Rate / immunology Rhinitis / immunology* Skin Tests |
| Chemical | |
Reg. No./Substance:
|
0/Allergens; 37341-29-0/Immunoglobulin E |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Improvement of toxic epidermal necrolysis after the early administration of a single high dose of in...
Next Document: Heteroaromatic monothiocarboxylic acids from Pseudomonas spp.