Document Detail


Occan, a novel transposon in the Fot1 family, is ubiquitously found in several Magnaporthe grisea isolates.
MedLine Citation:
PMID:  12612805     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated a DNA fragment and its flanking region deleted in the spontaneous Pi-a virulent mutant of Magnaporthe grisea Ina168. A new transposon-like sequence was identified from a region adjacent to the deleted fragment and was named Occan. Occan contained a 2,259-bp ORF interrupted by one 63-bp intron and had both a TA dinucleotide and 77 bp of perfect inverted repeats at both termini, without direct repeats. These features indicated that Occan is a member of the Fot1 family. RT-PCR analysis confirmed the expression of the putative transposase and the presence of an intron. Southern analysis of pulse-field gel electrophoresis-separated chromosomes indicated that Occan was dispersed in all chromosomes of the rice pathogen, Ina168. Copy numbers of Occan were also preserved in a host-specific manner amongst M. grisea isolates. In particular, rice pathogens contained a large number of the element inserted into their genome. Phylogenetic analysis with other known members of the Fot1 family revealed that Occan was dissimilar to any other known elements and it is thus proposed that Occan be separated to a new subfamily.
Authors:
Hideki Kito; Yosuke Takahashi; Junko Sato; Satoru Fukiya; Teruo Sone; Fusao Tomita
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Publication Detail:
Type:  Journal Article     Date:  2003-02-08
Journal Detail:
Title:  Current genetics     Volume:  42     ISSN:  0172-8083     ISO Abbreviation:  Curr. Genet.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-03     Completed Date:  2003-11-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8004904     Medline TA:  Curr Genet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  322-31     Citation Subset:  IM    
Affiliation:
Laboratory of Applied Microbiology, Department of Molecular Bioscience, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
DNA Transposable Elements*
Magnaporthe / genetics*
Molecular Sequence Data
Phylogeny
Random Amplified Polymorphic DNA Technique
Sequence Analysis, DNA
Sequence Homology
Transposases / genetics
Chemical
Reg. No./Substance:
0/DNA Transposable Elements; EC 2.7.7.-/Transposases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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