Document Detail


Observations on the results of specific histochemical techniques and empirical staining methods on several tissue/organ types using a variety of fixing fluids.
MedLine Citation:
PMID:  89068     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several histotechniques (Harris hematoxylin and alcoholic eosin, Gomori's reticulum method, Verhoeff's elastic stain; Gomori's trichrome, Mallory's aniline blue collagen stain and the Cameron and Steele method) were each used with a number of fixatives (Lavdowsky's chromic acid, Karnovsky's glutaraldehyde/paraformaldehyde, neutral buffered formalin, Lavdowsky's formalinacetic acid-alcohol, Zenker's and Helley's). Lavdowsky's chromic acid fixative provided superior results as compared to the other fixing fluids and its use was critical with respect to the trichrome or connective tissue staining procedures. The Cameron and Steele modification used with Lavdowsky's chromic acid fixative gave the best differentiation of tissue types and was also the most reproducible.
Authors:
W D Davenport; C R Ball
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Histopathology     Volume:  3     ISSN:  0309-0167     ISO Abbreviation:  Histopathology     Publication Date:  1979 Jul 
Date Detail:
Created Date:  1979-10-17     Completed Date:  1979-10-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7704136     Medline TA:  Histopathology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  321-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aging
Animals
Coronary Vessels / growth & development,  ultrastructure*
Histocytochemistry*
Mice
Staining and Labeling*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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