Document Detail

Observations on the nucleolar and total cell body nucleic acid of injured nerve cells.
MedLine Citation:
PMID:  5664236     Owner:  NLM     Status:  MEDLINE    
1. The nucleic acid content of neuronal nucleoli and the total cell body nucleic acid content of neurones of the hypoglossal nucleus were measured by ultraviolet absorption microspectrography.2. After nerve injury both the nucleolar nucleic acid and the total cell body nucleic acid increased: nucleolar changes preceded those of the cell body.3. The closer to the nerve cell body that the axon was injured the earlier was the onset and the decline of the nucleolar response.4. Actinomycin D was given to prevent DNA-primed RNA synthesis, and the rate of ;decay' of nucleolar RNA was measured. This rate varied after nerve injury and was closely related to the nucleolar nucleic acid content.5. The apparent rate of transfer of labelled RNA from the neuronal nucleus into the cytoplasm changed after nerve injury in a manner closely related to the changes in nucleolar nucleic acid content.6. It was demonstrated by making consecutive nerve injuries or by preventing or delaying nerve regeneration, that the nucleic acid changes were not induced by removal of contact between the neurone and its motor end-plate, and were not repressed by the restoration of such contact.7. When regeneration was prevented the nucleolar nucleic acid content and the total cell body nucleic acid ultimately decreased to values less than normal: this decrease was greater when more of the axon was initially removed.8. The results are discussed in relation to the factor responsible for derepression and repression of DNA cistrons for ribosome synthesis in injured nerve cells.
W E Watson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of physiology     Volume:  196     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  1968 Jun 
Date Detail:
Created Date:  1968-09-13     Completed Date:  1968-09-13     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  655-76     Citation Subset:  IM    
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MeSH Terms
Cell Nucleolus / analysis*
Cytoplasm / analysis
DNA / analysis
Dactinomycin / pharmacology
Hypoglossal Nerve / injuries
Medulla Oblongata / analysis
Molecular Biology
Nerve Regeneration
Neurons / analysis*,  injuries
Nucleic Acids / analysis*
RNA / analysis,  metabolism
Ribosomes / metabolism
Spectrum Analysis
Ultraviolet Rays
Reg. No./Substance:
0/Nucleic Acids; 50-76-0/Dactinomycin; 63231-63-0/RNA; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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