Document Detail

Observational study to determine factors associated with blood sample haemolysis in the emergency department.
MedLine Citation:
PMID:  18989489     Owner:  NLM     Status:  MEDLINE    
INTRODUCTION: Haemolysis of blood samples is a common problem encountered in the Emergency department (ED). It leads to inaccurate blood results and has cost implications as blood samples very often have to be retaken. The purpose of our study was to determine which factors in blood sampling were associated with higher rates of haemolysis. MATERIALS AND METHODS: An observational convenience sample of all patients presenting to the ED requiring blood urea and electrolyte (UE) analysis were eligible for our study. Questionnaires were distributed to the doctors and nurses conducting blood sampling to determine the method used and outcome data were collected after the samples were processed. RESULTS: Out of 227 UE samples analysed, 45 (19.8%) were haemolysed. Various factors, including method (IV cannulation or venepuncture), system (syringe or vacutainer), operator, rate of blood flow, difficulty of cannulation/venepuncture and source of blood (arterial or venous), were analysed, but their effects on haemolysis were not statistically significant (P >0.05). However, the use of the vacutainer system was associated with the highest rates of haemolysis [adjusted odds ratio (OR), 6.0; 95% confidence interval (CI), 2.3 to 15.1]. CONCLUSION: We found blood sampling with the vacutainer system to have increased rates of haemolysis. This could potentially change attitudes towards equipment used for blood sampling in the ED.
Marcus E H Ong; Yiong Huak Chan; Chin Siah Lim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annals of the Academy of Medicine, Singapore     Volume:  37     ISSN:  0304-4602     ISO Abbreviation:  Ann. Acad. Med. Singap.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-11-07     Completed Date:  2009-02-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7503289     Medline TA:  Ann Acad Med Singapore     Country:  Singapore    
Other Details:
Languages:  eng     Pagination:  745-8     Citation Subset:  IM    
Department of Emergency Medicine, Singapore General Hospital, Singapore.
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MeSH Terms
Catheters, Indwelling
Data Collection
Emergency Service, Hospital*
Phlebotomy / instrumentation*,  methods
Professional Competence
Syringes / utilization

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