| Observation on the morphological heterogeneity of WI-38 cells. | |
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MedLine Citation:
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PMID: 879045 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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WI-38 cells of intermediate and late-passage cultures were examined by light microscopy, scanning, conventional transmission and high voltage electron microscopy for evidence of heterogeneity among the cells within a single culture. Two morphologically distinct cell sizes and shapes were noted in all passages, (1) a typical fibroblastic type, and (2) a much larger, non-fusiform type. The larger cells generally had a nucleus that was positioned to one side of the bulk of the cytoplasm. The smaller was consistently fusiform with a centrally placed nucleus. The surfaces of intermediate-passage cells were uniform in showing small microvilli and corticall pits but were otherwise smooth and with out distinctive featues. The late-passage cells, on the other band, were consistent in showing numerous blebs and marginal ruffing. The internal structure of these cells in all passages studied was complicated by many age-related changes. The observations indicate that there is in WI-38 cells a high degree of intraculture heterogeneity. An awareness of this is important in studies which characterize the biochemical properties of this cell strain. |
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Authors:
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J J Wolosewick; K R Porter |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of anatomy Volume: 149 ISSN: 0002-9106 ISO Abbreviation: Am. J. Anat. Publication Date: 1977 Jun |
Date Detail:
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Created Date: 1977-08-12 Completed Date: 1977-08-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376312 Medline TA: Am J Anat Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 197-225 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line
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ultrastructure* Diploidy Fibroblasts / ultrastructure* Humans Lung / ultrastructure Microscopy, Electron Microscopy, Electron, Scanning |
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