| The observation of phenotypic changes of Schwann cells after rat sciatic nerve injury. | |
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MedLine Citation:
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PMID: 20047518 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To describe the phenotypic changes of Schwann cells during nerve regeneration, we made two different neurorrhaphy models after rat sciatic nerves injury, the epineurium neurorrhaphy and small gap bridging suture. Then, at selected time points after surgery (1d, 3d, 5d, 7d, 2 weeks, 3 weeks), the materials were drawn for detecting the expression of GFAP, Sox2, Krox20 by immunofluorescence. GFAP is expressed in non-myelin-forming Schwann cells and Krox20 is a marker for myelin-forming cells in the adult nerve. Sox2 is a marker for neural stem and progenitor cells. Our findings showed the rule of phenotypic changes of Schwann cells during nerve regeneration. Furthermore, the difference in the phenotypic changes of Schwann cells between two operation methods indicated that the small gap changes the regenerated microenvironment and may be one of the reasons that small gap bridging suture is superior to the epineurium suture. |
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Authors:
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Jin Wang; Peixun Zhang; Yanhua Wang; Yuhui Kou; Hongbo Zhang; Baoguo Jiang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Artificial cells, blood substitutes, and immobilization biotechnology Volume: 38 ISSN: 1532-4184 ISO Abbreviation: Artif Cells Blood Substit Immobil Biotechnol Publication Date: 2010 |
Date Detail:
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Created Date: 2010-01-19 Completed Date: 2010-06-10 Revised Date: 2010-09-07 |
Medline Journal Info:
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Nlm Unique ID: 9431307 Medline TA: Artif Cells Blood Substit Immobil Biotechnol Country: England |
Other Details:
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Languages: eng Pagination: 24-8 Citation Subset: IM |
Affiliation:
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Department of Trauma and Orthopedics, People's Hospital, Peking University, South Xi-Zhi-Men Street 11, Beijing, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anastomosis, Surgical* Animals Antigens, Differentiation / metabolism Cell Differentiation Early Growth Response Protein 2 / metabolism Glial Fibrillary Acidic Protein / metabolism Immunophenotyping Male Nerve Regeneration Peripheral Nerves / injuries, pathology*, surgery Rats Rats, Sprague-Dawley SOXB1 Transcription Factors / metabolism Schwann Cells / metabolism*, pathology* Sciatic Nerve / injuries, pathology*, surgery |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Differentiation; 0/Early Growth Response Protein 2; 0/Glial Fibrillary Acidic Protein; 0/SOXB1 Transcription Factors; 0/Sox2 protein, rat |
| Comments/Corrections | |
Erratum In:
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Artif Cells Blood Substit Immobil Biotechnol. 2010 Apr;38(2):113 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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