Document Detail


Obovatol isolated from Magnolia obovata enhances pentobarbital-induced sleeping time: Possible involvement of GABAA receptors/chloride channel activation.
MedLine Citation:
PMID:  19201178     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study aimed to investigate the effects of obovatol isolated from Magnolia obovata on pentobarbital-induced sleeping behaviors and to determine whether these effects were mediated by GABA(A) receptors/chloride channel activation, using a western blot technique and Cl(-) sensitive fluorescence probe. GABA(A) receptors subunits expression and chloride influx were investigated in cultured cerebellar granule cells. Obovatol (0.05, 0.1, and 0.2 mg/kg) prolonged the sleeping time induced by pentobarbital (42 mg/kg). In addition, obovatol (20 and 50 microM) significantly increased Cl(-) influx in the primary cultured cerebellar granule cells. Moreover, obovatol increased the expression of GABA(A) receptor alpha-, beta-, and gamma-subunits. However, it had no effect on the abundance of the expression of glutamic acid decarboxylase (GAD), suggesting that obovatol might not activate GAD. These results suggest that obovatol potentiates pentobarbital-induced sleeping time through the GABA(A) receptors/chloride channel activation.
Authors:
Hong Ma; Young-Jun Jo; Yuan Ma; Jin-Tae Hong; Byoung-Mog Kwon; Ki-Wan Oh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-06
Journal Detail:
Title:  Phytomedicine : international journal of phytotherapy and phytopharmacology     Volume:  16     ISSN:  1618-095X     ISO Abbreviation:  Phytomedicine     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-24     Completed Date:  2009-05-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438794     Medline TA:  Phytomedicine     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  308-13     Citation Subset:  IM    
Affiliation:
College of Pharmacy, Chungbuk National University, Cheongju 361-763, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Behavior, Animal / drug effects
Biphenyl Compounds / chemistry,  isolation & purification,  pharmacology*
Brain / metabolism*
Chloride Channels / metabolism*
GABA Modulators / pharmacology*
Glutamate Decarboxylase / metabolism
Magnolia* / chemistry
Male
Mice
Mice, Inbred ICR
Neurons / metabolism
Pentobarbital / pharmacology
Phenyl Ethers / chemistry,  isolation & purification,  pharmacology*
Plant Extracts / pharmacology
Plant Leaves
Receptors, GABA-A / metabolism*
Sleep / drug effects*
Chemical
Reg. No./Substance:
0/Biphenyl Compounds; 0/Chloride Channels; 0/GABA Modulators; 0/Phenyl Ethers; 0/Plant Extracts; 0/Receptors, GABA-A; 76-74-4/Pentobarbital; 83864-78-2/obovatol; EC 4.1.1.15/Glutamate Decarboxylase; EC 4.1.1.15/glutamate decarboxylase 1; EC 4.1.1.15/glutamate decarboxylase 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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