| Obesity and type 2 diabetes in rats are associated with altered brain glycogen and amino-acid homeostasis. | |
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MedLine Citation:
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PMID: 20424632 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Obesity and type 2 diabetes have reached epidemic proportions; however, scarce information about how these metabolic syndromes influence brain energy and neurotransmitter homeostasis exist. The objective of this study was to elucidate how brain glycogen and neurotransmitter homeostasis are affected by these conditions. [1-(13)C]glucose was administered to Zucker obese (ZO) and Zucker diabetic fatty (ZDF) rats. Sprague-Dawley (SprD), Zucker lean (ZL), and ZDF lean rats were used as controls. Several brain regions were analyzed for glycogen levels along with (13)C-labeling and content of glutamate, glutamine, GABA, aspartate, and alanine. Blood glucose concentrations and (13)C enrichment were determined. (13)C-labeling in glutamate was lower in ZO and ZDF rats in comparison with the controls. The molecular carbon labeling (MCL) ratio between alanine and glutamate was higher in the ZDF rats. The MCL ratios of glutamine and glutamate were decreased in the cerebellum of the ZO and the ZDF rats. Glycogen levels were also lower in this region. These results suggest that the obese and type 2 diabetic models were associated with lower brain glucose metabolism. Glucose metabolism through the TCA cycle was more decreased than glycolytic activity. Furthermore, reduced glutamate-glutamine cycling was also observed in the obese and type 2 diabetic states. |
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Authors:
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Helle M Sickmann; Helle S Waagepetersen; Arne Schousboe; Andrew J Benie; Stephan D Bouman |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-28 |
Journal Detail:
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Title: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Volume: 30 ISSN: 1559-7016 ISO Abbreviation: J. Cereb. Blood Flow Metab. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-03 Completed Date: 2010-09-07 Revised Date: 2011-08-03 |
Medline Journal Info:
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Nlm Unique ID: 8112566 Medline TA: J Cereb Blood Flow Metab Country: United States |
Other Details:
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Languages: eng Pagination: 1527-37 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Pharmacotherapy, Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, Denmark. hms@farma.ku.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alanine
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metabolism Amino Acids / metabolism* Animals Aspartic Acid / metabolism Blood Glucose / metabolism Brain / metabolism* Diabetes Mellitus, Type 2 / metabolism* Glutamic Acid / metabolism Glutamine / metabolism Glycogen / metabolism* Male Obesity / metabolism* Rats Rats, Sprague-Dawley Rats, Zucker gamma-Aminobutyric Acid / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Blood Glucose; 56-12-2/gamma-Aminobutyric Acid; 56-41-7/Alanine; 56-84-8/Aspartic Acid; 56-85-9/Glutamine; 56-86-0/Glutamic Acid; 9005-79-2/Glycogen |
| Comments/Corrections | |
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