Document Detail

Obesity, insulin resistance, and skeletal muscle nitric oxide synthase.
MedLine Citation:
PMID:  22797309     Owner:  NLM     Status:  MEDLINE    
The molecular mechanisms responsible for impaired insulin action have yet to be fully identified. Rodent models demonstrate a strong relationship between insulin resistance and an elevation in skeletal muscle inducible nitric oxide synthase (iNOS) expression; the purpose of this investigation was to explore this potential relationship in humans. Sedentary men and women were recruited to participate (means ± SE: nonobese, body mass index = 25.5 ± 0.3 kg/m(2), n = 13; obese, body mass index = 36.6 ± 0.4 kg/m(2), n = 14). Insulin sensitivity was measured using an intravenous glucose tolerance test with the subsequent modeling of an insulin sensitivity index (S(I)). Skeletal muscle was obtained from the vastus lateralis, and iNOS, endothelial nitric oxide synthase (eNOS), and neuronal nitric oxide synthase (nNOS) content were determined by Western blot. S(I) was significantly lower in the obese compared with the nonobese group (~43%; P < 0.05), yet skeletal muscle iNOS protein expression was not different between nonobese and obese groups. Skeletal muscle eNOS protein was significantly higher in the nonobese than the obese group, and skeletal muscle nNOS protein tended to be higher (P = 0.054) in the obese compared with the nonobese group. Alternative analysis based on S(I) (high and low tertile) indicated that the most insulin-resistant group did not have significantly more skeletal muscle iNOS protein than the most insulin-sensitive group. In conclusion, human insulin resistance does not appear to be associated with an elevation in skeletal muscle iNOS protein in middle-aged individuals under fasting conditions.
Raymond M Kraus; Joseph A Houmard; William E Kraus; Charles J Tanner; Joseph R Pierce; Myung Dong Choi; Robert C Hickner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-07-12
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  113     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-03     Completed Date:  2013-07-08     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  758-65     Citation Subset:  IM    
Department of Kinesiology, East Carolina University, Greenville, North Carolina 27858, USA.
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MeSH Terms
Blood Glucose / metabolism
Cohort Studies
Exercise Test / methods
Insulin Resistance / physiology*
Middle Aged
Muscle, Skeletal / enzymology*
Nitric Oxide Synthase / blood,  metabolism
Nitric Oxide Synthase Type I / blood,  metabolism*
Nitric Oxide Synthase Type II / blood,  metabolism*
Nitric Oxide Synthase Type III / blood,  metabolism*
Obesity / blood,  enzymology*
Grant Support
R01 HL-57354/HL/NHLBI NIH HHS; R21 AG-19209-02/AG/NIA NIH HHS
Reg. No./Substance:
0/Blood Glucose; EC protein, human; EC protein, human; EC Oxide Synthase; EC Oxide Synthase Type I; EC Oxide Synthase Type II; EC Oxide Synthase Type III

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