| Obesity improves myocardial ischaemic tolerance and RISK signalling in insulin-insensitive rats. | |
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MedLine Citation:
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PMID: 23161371 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Obesity with associated metabolic disturbances worsens ischaemic heart disease outcomes, and rodent studies confirm obesity with insulin-resistance impairs myocardial resistance to ischemia-reperfusion (I-R) injury. However, effects of obesity per se are unclear, with some evidence for paradoxic cardioprotection (particularly in older subjects). We tested the impact of dietary obesity on I-R tolerance and reperfusion injury salvage kinase (RISK) signalling in hearts from middle-aged (10 month) insulin-insensitive rats. Hearts from Wistar rats on 32 week control (CD) or high carbohydrate obesogenic (OB) diets were assessed for I-R resistance in vivo (45 min left anterior descending artery occlusion/120 min reperfusion) and ex vivo (25 min ischemia/60 min reperfusion), with expression and δ-opioid receptor (δ-OR) phospho-regulation of pro-survival (Akt/PKB, Erk1/2, eNOS) and pro-injury (GSK3β) enzymes also examined. OB rats were heavier (764±25 vs. 657±22 g for CD; P<0.05), hyperleptinaemic (11.1±0.7 vs. 5.0±0.7; P<0.01), and comparably insulin-insensitive (HOMA-IR = 63.2±3.3 vs. 63.2±1.6 for CD). In vivo infarction was more than halved in OB (20±3%) vs. CD rats (45±6% P<0.05), as was post-ischaemic LDH efflux (0.4±0.3 mU/ml vs. 5.6±0.5 mU/ml; P<0.02) and ex vivo contractile dysfunction (62±2% vs. 44±6% recovery of ventricular force; P<0.05). OB hearts exhibited up to 60% higher Akt expression, with increased phosphorylation of eNOS (+100%), GSK3ß (+45%), and Erk1/2 (+15%). Pre-ischaemic δ-OR agonism (1 µM BW373U86) improved recoveries in CD hearts in association with phosphorylation of Akt (+40%), eNOS (+75%) and GSK3ß (+30%), yet failed to further enhance RISK/NOS activation or I-R outcomes in OB hearts. In summary, dietary obesity in the context of age-related insulin-insensitivity paradoxically improves myocardial I-R tolerance, in association with moderate hyperleptinaemic and enhanced RISK expression and phospho-regulation. However, OB hearts are resistant to further RISK modulation and cardioprotection via acute δ-OR agonism. |
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Authors:
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Daniel G Donner; John P Headrick; Jason N Peart; Eugene F Du Toit |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-16 |
Journal Detail:
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Title: Disease models & mechanisms Volume: - ISSN: 1754-8411 ISO Abbreviation: Dis Model Mech Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101483332 Medline TA: Dis Model Mech Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Griffith University , QLD, Australia; |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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